Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract

Glavinić, Uroš (author), Dzogovic, Danica (author), Jelisic, Stefan (author), Ristanić, Marko (author), Zorc, Minja (author), Aleksic, Nevenka (author), Stanimirović, Zoran (author)

Nosema ceranae, a microsporidium species, is among the most common causes of bee diseases. The positive effect of Agaricus bisporus mushroom extract on the survival and immunity of Nosema-infected bees has been reported recently. The effect could be achieved by stimulating the expression of immune-related genes, but also by suppressing nosemosis. The aim of this work was to determine the effect of A. bisporus extract on the oxidative status of bees infected with N. ceranae. In a cage experiment on newly hatched bees, the effect of aqueous extract of champignon (A. bisporus, strain A15) was investigated. Six groups were formed: three groups were infected and received A. bisporus extract through food at different times (days 1, 3, and 6 after hatching), one group received the extract but was not infected (treatment control), one was only infected with Nosema (positive control) and one was neither infected nor received the extract (negative control). The effects were examined on samples taken on days 7 and 15 of the study. The activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) and the concentrations of malondialdehyde (MDA) were determined. In comparison to the positive control, the enzyme activities and MDA concentrations were significantly lower in the groups fed with the mushroom extract supplement. In the negative control, the level of oxidative stress was lower than in the positive control. In comparison with the other groups, the values mostly did not differ significantly. The oxidative status of bees infected with N. ceranae was significantly better if they were fed with the A. bisporus extract.

• Language: English
• Keywords: Apis mellifera, Nosema ceranae, Agaricus bisporus, oxidative stress, oxidative status
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: Str. 35-50
• Numbering: Vol. 77, iss. 1
• PID: 20.500.12556/RUL-147381
• UDC: 638.1
• ISSN on article: 2406-0771
• DOI: 10.2298/VETGL220715013G
• COBISS.SI-ID: 130012419
• Publication date in RUL: 03.07.2023

Record is a part of a journal

• Title: Veterinarski glasnik
• Shortened title: Vet. glas.
• Publisher: Veterinarski fakultet
• ISSN: 2406-0771
• COBISS.SI-ID: 526252569

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Serbian (cyrillic)
• Title: Oksidativni stres pčela zaraženih mikosporidijom Nosema ceranae i tretiranih ekstraktom gljive Agaricus bisporus

Abstract

Među najučestalije izazivače oboljenja pčela spada mikrosporidija Nosema ceranae. Poznato je pozitivno dejstvo ekstrakta gljive Agaricus bisporus na preživljavanje i imunitet pčela inficiranih nozemom. To se postiže stimulacijom ekspresije gena značajnih za imunitet i suzbijanjem nozemoze. Cilj ovog rada bio je utvrditi kakav efekat ima ekstrakt šampinjona A. bisporus na oksidativni status pčela inficiranih mikrosporidijom N. ceranae. U kaveznom eksperimentu na novoizleženim pčelama ispitivan je efekat vodenog ekstrakta šampinjona (A. bisporus, soj A15). Oformljeno je šest grupa: tri grupe su bile inficirane i u hrani dobijale ekstrakt A. bisporus u različitim momentima (prvog, trećeg i šestog dana od izleganja), jedna grupa je dobijala ekstrakt, ali nije bila inficirana (kontrola tretmana), jedna je bila samo inficirana nozemom (pozitivna kontrola) i jedna nije bila inficirana niti je dobijala ekstrakt (negativna kontrola). Efekti su ispitivani na uzorcima uzetim 7. i 15. dana eksperimenta. Određene su aktivnosti antioksidativnih enzima katalaze (CAT), superoksid dismutaze (SOD) i glutation S-transferaze (GST), kao i koncentracija malonaldehida (MDA). Aktivnosti enzima i koncentracija malonaldehida bile su značajno niže kod grupa koje su dobijale ekstrakt šampinjona u poređenju sa pozitivnom kontrolom. Kod pčela iz negativne kontrole zabeležen je niži nivo oksidativnog stresa u odnosu na pozitivnu kontrolu, a u odnosu na druge grupe, vrednosti se uglavnom nisu nisu značajno razlikovale. Oksidativni status pčela inficiranih mikrosporidijom N. ceranae bio je značajno bolji ukoliko su one prihranjivane ekstraktom gljive A. bisporus.

• Keywords: čebelarstvo, čebele, bolezni, okužbe, Nosema ceranae, glive, Agaricus bisporus, oksidativni status

Projects

• Funder: Other - Other funder or multiple funders
• Funding programme: Ministry of Education, Science and Technological Development of the Republic of Serbia
• Project number: 451-03-68/2022-14/200143