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eDNA provides a scaffold for autoaggregation of B. subtilis in bacterioplankton suspension
ID Dogša, Iztok (Author), ID Kostanjšek, Rok (Author), ID Stopar, David (Author)

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Abstract
The self-binding of bacterial cells, or autoaggregation, is, together with surface colonization, one of the first steps in the formation of a mature biofilm. In this work, the autoaggregation of B. subtilis in dilute bacterial suspensions was studied. The dynamics of cell lysis, eDNA release, and bacterial autoaggregate assembly were determined and related to the spatial autocorrelation of bacterial cells in dilute planktonic bacterial suspensions. The non-random distribution of cells was associated with an eDNA network, which stabilized the initial bacterial cell-cell aggregates. Upon the addition of DNase I, the aggregates were dispersed. The release of eDNA during cell lysis allows for the entrapment of bacterial drifters at a radius several times the size of the dying bacteria. The size of bacterial aggregates increased from 2 to about 100 μm in diameter in dilute bacterial suspensions. The results suggest that B. subtilis cells form previously unnoticed continuum of autoaggregate structures during planktonic growth.

Language:English
Keywords:autoaggregates, B. subtilis, plankton, eDNA, biofilm
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Year:2023
Number of pages:15 str.
Numbering:Vol. 11, iss. 2, art. 332
PID:20.500.12556/RUL-145742 This link opens in a new window
UDC:579.25:579.852.11
ISSN on article:2076-2607
DOI:10.3390/microorganisms11020332 This link opens in a new window
COBISS.SI-ID:142263043 This link opens in a new window
Publication date in RUL:11.05.2023
Views:225
Downloads:35
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Record is a part of a journal

Title:Microorganisms
Shortened title:Microorganisms
Publisher:MDPI AG
ISSN:2076-2607
COBISS.SI-ID:523277081 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:avtoagregacija, eDNA, bakterijski plankton, Bacillus subtilis, biofilm

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P4-0116
Name:Mikrobiologija in biotehnologija živil in okolja

Funder:ARRS - Slovenian Research Agency
Project number:J1-3021
Name:Platforma osnovana na sintetičnih biofilmih za preučevanje in razvoj novih proti biofilmskih pristopov

Funder:ARRS - Slovenian Research Agency
Project number:L7-3186
Name:Lasersko podprto odstranjevanje biofilmov pri parodontalnih in periimplantnih boleznih

Funder:ARRS - Slovenian Research Agency
Project number:I0-0022
Name:Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)

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