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Ceramide phosphoethanolamine as a possible marker of periodontal disease
ID Grundner, Maja (Author), ID Munjaković, Haris (Author), ID Tori, Tilen (Author), ID Sepčić, Kristina (Author), ID Gašperšič, Rok (Author), ID Oblak, Čedomir (Author), ID Seme, Katja (Author), ID Guella, Graziano (Author), ID Trenti, Francesco (Author), ID Skočaj, Matej (Author)

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Abstract
Periodontal disease is a chronic oral inflammatory disorder initiated by pathobiontic bacteria found in dental plaques—complex biofilms on the tooth surface. The disease begins as an acute local inflammation of the gingival tissue (gingivitis) and can progress to periodontitis, which eventually leads to the formation of periodontal pockets and ultimately results in tooth loss. The main problem in periodontology is that the diagnosis is based on the assessment of the already obvious tissue damage. Therefore, it is necessary to improve the current diagnostics used to assess periodontal disease. Using lipidomic analyses, we show that both crucial periodontal pathogens, Porphyromonas gingivalis and Tannerella forsythia, synthesize ceramide phosphoethanolamine (CPE) species, membrane sphingolipids not typically found in vertebrates. Previously, it was shown that this particular lipid can be specifically detected by an aegerolysin protein, erylysin A (EryA). Here, we show that EryA can specifically bind to CPE species from the total lipid extract from P. gingivalis. Furthermore, using a fluorescently labelled EryA-mCherry, we were able to detect CPE species in clinical samples of dental plaque from periodontal patients. These results demonstrate the potential of specific periodontal pathogen-derived lipids as biomarkers for periodontal disease and other chronic inflammatory diseases.

Language:English
Keywords:ceramide phosphoethanolamine, periodontal disease, aegerolysin, biomarker, erylysin A, Porphyromonas gingivalis
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
MF - Faculty of Medicine
Publication status:Published
Publication version:Version of Record
Year:2022
Number of pages:19 str.
Numbering:Vol. 12, iss. 7, art. 655
PID:20.500.12556/RUL-145170 This link opens in a new window
UDC:616.31
ISSN on article:2077-0375
DOI:10.3390/membranes12070655 This link opens in a new window
COBISS.SI-ID:113667587 This link opens in a new window
Publication date in RUL:12.04.2023
Views:436
Downloads:56
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Record is a part of a journal

Title:Membranes
Shortened title:Membranes
Publisher:MDPI
ISSN:2077-0375
COBISS.SI-ID:519125529 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:ceramid fosfoetanolamin, parodontalna bolezen, egerolizin

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P1-0207
Name:Toksini in biomembrane

Funder:ARRS - Slovenian Research Agency
Project number:J3-1747
Name:Egerolizinski proteini kot nova teranostična sredstva pri parodontalni bolezni in protetični oskrbi

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