Ceramide phosphoethanolamine as a possible marker of periodontal disease

Grundner, Maja (author), Munjaković, Haris (author), Tori, Tilen (author), Sepčić, Kristina (author), Gašperšič, Rok (author), Oblak, Čedomir (author), Seme, Katja (author), Guella, Graziano (author), Trenti, Francesco (author), Skočaj, Matej (author)

Periodontal disease is a chronic oral inflammatory disorder initiated by pathobiontic bacteria found in dental plaques—complex biofilms on the tooth surface. The disease begins as an acute local inflammation of the gingival tissue (gingivitis) and can progress to periodontitis, which eventually leads to the formation of periodontal pockets and ultimately results in tooth loss. The main problem in periodontology is that the diagnosis is based on the assessment of the already obvious tissue damage. Therefore, it is necessary to improve the current diagnostics used to assess periodontal disease. Using lipidomic analyses, we show that both crucial periodontal pathogens, Porphyromonas gingivalis and Tannerella forsythia, synthesize ceramide phosphoethanolamine (CPE) species, membrane sphingolipids not typically found in vertebrates. Previously, it was shown that this particular lipid can be specifically detected by an aegerolysin protein, erylysin A (EryA). Here, we show that EryA can specifically bind to CPE species from the total lipid extract from P. gingivalis. Furthermore, using a fluorescently labelled EryA-mCherry, we were able to detect CPE species in clinical samples of dental plaque from periodontal patients. These results demonstrate the potential of specific periodontal pathogen-derived lipids as biomarkers for periodontal disease and other chronic inflammatory diseases.

• Language: English
• Keywords: ceramide phosphoethanolamine, periodontal disease, aegerolysin, biomarker, erylysin A, Porphyromonas gingivalis
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty; MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 19 str.
• Numbering: Vol. 12, iss. 7, art. 655
• PID: 20.500.12556/RUL-145170
• UDC: 616.31
• ISSN on article: 2077-0375
• DOI: 10.3390/membranes12070655
• COBISS.SI-ID: 113667587
• Publication date in RUL: 12.04.2023

Record is a part of a journal

• Title: Membranes
• Shortened title: Membranes
• Publisher: MDPI
• ISSN: 2077-0375
• COBISS.SI-ID: 519125529

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: ceramid fosfoetanolamin, parodontalna bolezen, egerolizin

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0207
• Name: Toksini in biomembrane

• Funder: ARRS - Slovenian Research Agency
• Project number: J3-1747
• Name: Egerolizinski proteini kot nova teranostična sredstva pri parodontalni bolezni in protetični oskrbi