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Novel identification of the collection of pathogenic fungal species Verticillium with the development of species-specific SSR markers
ID
Jeseničnik, Taja
(
Author
),
ID
Kaurin, Anela
(
Author
),
ID
Grgič, Zarja
(
Author
),
ID
Radišek, Sebastjan
(
Author
),
ID
Jakše, Jernej
(
Author
),
ID
Štajner, Nataša
(
Author
)
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https://www.mdpi.com/2076-0817/12/4/535
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Abstract
The genus Verticillium is a group of ascomycete fungi that includes several pathogenic plant species. In 2011, a new taxonomic classification, proposed by Inderbitzin and coworkers (2011), re-defined the genus as Verticillium sensu stricto. The objective of our study was the re-classification of the fungal species held in the culture collection in the Slovenian Institute of Hop Research and Brewing in accordance with the newly established taxonomy. With the PCR marker system proposed by Inderbitzin and coworkers in 2011, we re-classified 88 Verticillium isolates out of the 105 samples that are held in the institute’s bank, which were obtained from different geographic locations in Europe, North America, and Japan, and from different host plants, including alfalfa, cotton, hop, olive, potato, and tomato. However, the PCR marker for the V. dahliae identification proved to be less specific, and it resulted in the positive amplification of Gibellulopsis nigrescens, V. isaacii, and V. longisporum. To enable the accurate distinction of the fungi, the SSR and LAMP markers were added to the analyses. The 12 newly identified SSR markers, which were used in simplex PCR reactions or in combination, enabled the accurate identification of all included Verticillium isolates and could potentially be used as biomarkers for rapid and easy species identification.
Language:
English
Keywords:
classification
,
LAMP
,
multiplex PCR
,
SSR marker
,
Vericillium
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
BF - Biotechnical Faculty
Year:
2023
Number of pages:
18 str.
Numbering:
Vol. 12, no. 4, art. 535
PID:
20.500.12556/RUL-145021
UDC:
577.2
ISSN on article:
2076-0817
COBISS.SI-ID:
147144451
Publication date in RUL:
30.03.2023
Views:
461
Downloads:
72
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Record is a part of a journal
Title:
Pathogens
Shortened title:
Pathogens
Publisher:
MDPI
ISSN:
2076-0817
COBISS.SI-ID:
523338009
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
glive
,
Verticillium
,
identifikacija
,
klasifikacija
,
SSR markerji
,
PCR
Projects
Funder:
ARRS - Slovenian Research Agency
Funding programme:
Young Researchers
Project number:
34110
Funder:
ARRS - Slovenian Research Agency
Project number:
P4-0077
Name:
Kmetijske rastline - genetika in sodobne tehnologije
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