Novel identification of the collection of pathogenic fungal species Verticillium with the development of species-specific SSR markers

Jeseničnik, Taja (author), Kaurin, Anela (author), Grgič, Zarja (author), Radišek, Sebastjan (author), Jakše, Jernej (author), Štajner, Nataša (author)

The genus Verticillium is a group of ascomycete fungi that includes several pathogenic plant species. In 2011, a new taxonomic classification, proposed by Inderbitzin and coworkers (2011), re-defined the genus as Verticillium sensu stricto. The objective of our study was the re-classification of the fungal species held in the culture collection in the Slovenian Institute of Hop Research and Brewing in accordance with the newly established taxonomy. With the PCR marker system proposed by Inderbitzin and coworkers in 2011, we re-classified 88 Verticillium isolates out of the 105 samples that are held in the institute’s bank, which were obtained from different geographic locations in Europe, North America, and Japan, and from different host plants, including alfalfa, cotton, hop, olive, potato, and tomato. However, the PCR marker for the V. dahliae identification proved to be less specific, and it resulted in the positive amplification of Gibellulopsis nigrescens, V. isaacii, and V. longisporum. To enable the accurate distinction of the fungi, the SSR and LAMP markers were added to the analyses. The 12 newly identified SSR markers, which were used in simplex PCR reactions or in combination, enabled the accurate identification of all included Verticillium isolates and could potentially be used as biomarkers for rapid and easy species identification.

• Language: English
• Keywords: classification, LAMP, multiplex PCR, SSR marker, Vericillium
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 2023
• Number of pages: 18 str.
• Numbering: Vol. 12, no. 4, art. 535
• PID: 20.500.12556/RUL-145021
• UDC: 577.2
• ISSN on article: 2076-0817
• COBISS.SI-ID: 147144451
• Publication date in RUL: 30.03.2023

Record is a part of a journal

• Title: Pathogens
• Shortened title: Pathogens
• Publisher: MDPI
• ISSN: 2076-0817
• COBISS.SI-ID: 523338009

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: glive, Verticillium, identifikacija, klasifikacija, SSR markerji, PCR

Projects

• Funder: ARRS - Slovenian Research Agency
• Funding programme: Young Researchers
• Project number: 34110

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0077
• Name: Kmetijske rastline - genetika in sodobne tehnologije