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Vpliv pozebe na vsebnosti glukozinolatov in sladkorjev v genskih virih listnega ohrovta (Brassica oleracea L.)
ID Uranič, Živa (Author), ID Jakopič, Jerneja (Mentor) More about this mentor... This link opens in a new window, ID Sinkovič, Lovro (Comentor)

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Abstract
Listni ohrovt (Brassica oleracea L.) spada v družino križnic in je dvoletna rastlina, ki se jo goji po vsem svetu. Priljubljen je zaradi nezahtevnosti za gojenje, saj uspeva tudi na manj rodovitnih tleh in je toleranten na nizke temperature. Pomemben je v prehrani, saj so listi bogati s hranili in bioaktivnimi snovmi, kot so vitamini, minerali, glukozinolati in fenolne spojine. V magistrski nalogi smo želeli identificirati in kvantificirati vsebnosti sladkorjev in glukozinolatov v rastlinah listnega ohrovta in ugotoviti kakšne so razlike v vsebnosti le-teh med različnimi genskimi viri listnega ohrovta. Prav tako smo poskušali ugotoviti ali obstajajo razlike v vsebnosti sladkorjev in glukozinolatov pri nepozeblih in pozeblih rastlinah. Ugotovili smo, da so vsi genski viri listnega ohrovta vsebovali štiri sladkorje, in sicer saharozo, glukozo, fruktozo in galaktozo. Vsebnosti sladkorjev so se značilno razlikovale med pozeblimi in ne pozeblimi rastlinami. Vsebnost skupnih sladkorjev pred pozebo je bila od 201,2 do 338,1 g/kg suhe mase (DW), po pozebi pa od 223,9 do 388,3 g/kg DW. Identificirali smo enajst glukozinolatov, ki smo jih razdelili v tri skupine. Med alifatskimi glukozinolati so bili prisotni glukobrasikanapin, sinigrin, glukonapin, glukoiberin ter glukorafanin in progoitrin pri katerima je bila vsebnost pod mejo detekcije. Med indolnimi glukozinolati so bili prisotni glukobrasicin, 4-hidroksiglukobrasicin, 4-metoksiglukobrasicin in neoglukobrasicin, pri katerem so bile vsebnosti pod mejo detekcije. Med aromatskimi glukozinolati je bil prisoten glukonasturtiin. Vsebnosti večine glukozinolatov so se pri ne pozeblih in pozeblih rastlinah značilno razlikovale, pri nekaterih genskih virih listnega ohrovta pa razlik med ne pozeblimi in pozeblimi rastlinami ni bilo mogoče dokazati.

Language:Slovenian
Keywords:listni ohrovt, rastlinski genski viri, sladkorji, glukozinolati, pozeba
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2023
PID:20.500.12556/RUL-144968 This link opens in a new window
COBISS.SI-ID:146712067 This link opens in a new window
Publication date in RUL:26.03.2023
Views:727
Downloads:118
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Secondary language

Language:English
Title:The effect of the frost on the content of glucosinolates and sugars in the genetic sources of kale (Brassica oleracea L.)
Abstract:
Leafy kale (Brassica oleracea L.) is a biannual leafy vegetable, of the Brassicacea family that is cultivated all over the world. It is popular for it`s undemanding cultivation, as it can even be grown on less fertile soil and is tolerant on low temperatures and frost. Leafy kale is important nutritional food, because it is rich in nutrients and bioactive substances as vitamins, minerals, glucosinolates and phenolic compounds. In the Master`s thesis, we wanted to identify sugars and glucosinolates in leafy kale plants and to determine the differences in their content among different leafy kale genetic resources. We also tried to find out if there are differences in the content of sugars and glucosinolates among plants grown in optimal conditions and plants exposed to frost. We found out that all genetic resources of leafy kale contained four sugars: sucrose, glucose, fructose and galactose. Sugar content significantly differed between frosted and non-frosted plants. Total sugar content was from 201,2 to 338,1 g/kg dry weight (DW) before frost and from 223,9 to 388,3 g/kg DW after frost. We identified eleven glucosinolates, which were divided into three groups. Glucobrassicanapin, sinigrin, gluconapin, glucoiberin, glucoraphanin and progoitrin were present among the aliphatic glucosinolates. Glucobrassicin, 4-hydroxyglucobrassicin and neoglucobrassicin were presented among the indole glucosinolates and gluconasturtiin was presentend among aromatic glucosinolates. The contents of most glucosinolates significantly differed among non-frosted and frosted plants, however for some glucosinolates the difference between non-frosted and frosted palnts could not ne demonstrated.

Keywords:leafy kale, plant genetic resources, sugars, glucosinolates, frost

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