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Detrimental effect of various preparations of the human amniotic membrane homogenate on the 2D and 3D bladder cancer in vitro models
ID
Janev, Aleksandar
(
Author
),
ID
Železnik Ramuta, Taja
(
Author
),
ID
Tratnjek, Larisa
(
Author
),
ID
Sardoč, Žiga
(
Author
),
ID
Obradović, Hristina
(
Author
),
ID
Mojsilović, Slavko
(
Author
),
ID
Taskovska, Milena
(
Author
),
ID
Smrkolj, Tomaž
(
Author
),
ID
Erdani-Kreft, Mateja
(
Author
)
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https://www.frontiersin.org/articles/10.3389/fbioe.2021.690358/full
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Abstract
Despite being among the ten most common cancers with high recurrence rates worldwide, there have been no major breakthroughs in the standard treatment options for bladder cancer in recent years. The use of a human amniotic membrane (hAM) to treat cancer is one of the promising ideas that have emerged in recent years. This study aimed to investigate the anticancer activity of hAM homogenate on 2D and 3D cancer models. We evaluated the effects of hAM homogenates on the human muscle invasive bladder cancer urothelial (T24) cells, papillary cancer urothelial (RT4) cells and normal porcine urothelial (NPU) cells as well as on human mammary gland nontumorigenic (MCF10a) cells and low-metastatic breast cancer (MCF7) cells. After 24 h, we observed a gradual detachment of cancerous cells from the culture surface, while the hAM homogenate did not affect the normal cells. The most pronounced effect hAM homogenate had on bladder cancer cells; however, the potency of their detachment was dependent on the treatment protocol and the preparation of hAM homogenate. We demonstrated that hAM homogenate significantly decreased the adhesion, growth, and proliferation of human bladder invasive and papillary cancer urothelial cells and did not affect normal urothelial cells even in 7-day treatment. By using light and electron microscopy we showed that hAM homogenate disrupted the architecture of 2D and 3D bladder cancer models. The information provided by our study highlights the detrimental effect of hAM homogenate on bladder cancer cells and strengthens the idea of the potential clinical application of hAM for bladder cancer treatment.
Language:
English
Keywords:
cancer
,
urothelium
,
2D and 3D in vitro models
,
light and electron microscopy
,
proliferation
,
cell cycle
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Year:
2021
Number of pages:
19 str.
Numbering:
Vol. 9, art. 690358
PID:
20.500.12556/RUL-144672
UDC:
616.6
ISSN on article:
2296-4185
DOI:
10.3389/fbioe.2021.690358
COBISS.SI-ID:
69118467
Publication date in RUL:
07.03.2023
Views:
1124
Downloads:
52
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Record is a part of a journal
Title:
Frontiers in bioengineering and biotechnology
Shortened title:
Front. bioeng. biotechnol.
Publisher:
Frontiers Media
ISSN:
2296-4185
COBISS.SI-ID:
523093017
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
rak sečnega mehurja
,
urotelij
,
2D in 3D in vitro modeli
,
elektronski mikroskop
,
proliferacija
,
urologija
Projects
Funder:
ARRS - Slovenian Research Agency
Funding programme:
Young researchers
Funder:
ARRS - Slovenian Research Agency
Project number:
J7-2594
Name:
Uporaba amnijske membrane za inovativno multimodalno zdravljenje bakterijskega cistitisa in raka sečnega mehurja: učinek njene protimikrobne, imunomodulatorne in protirakave aktivnosti
Funder:
ARRS - Slovenian Research Agency
Project number:
P3-0108
Name:
Diferenciacija urotelijskih celic
Funder:
ARRS - Slovenian Research Agency
Project number:
I0-0022
Name:
Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)
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