Advances in physicochemical and biochemical characterization of archaeosomes from polar lipids of Aeropyrum pernix K1 and stability in biological systems

Kejžar, Jan (author), Osojnik Črnivec, Ilja Gasan (author), Poklar Ulrih, Nataša (author)

Archaeosomes are vesicles made from archaeal lipids. They are characterized by remarkable thermostability, resistance to enzymatic degradation, long-term stability, and immunomodulatory properties. In this review the current status of physicochemical properties of archaeal lipids and their stability in biological systems is presented, focusing on total polar lipids from Aeropyrum pernix K1. The isolated total polar lipids from Aeropyrum pernix K1 consist exclusively of glycerol ether lipids with isoprenoid groups attached to glycerol via ether linkages. More specifically, the two major polar lipids extracted from the membranes are C$_{25,25}$-achaetidyl(glucosyl)inositol and C$_{25,25}$-achaetidylinositol. An overview of the results of the effects of temperature and pH on the stability, structural organization, fluidity, and permeability of archaeosomes composed of pure C$_{25,25}$ was examined by a combination of techniques, including fluorescence emission spectroscopy, electron paramagnetic resonance, differential scanning calorimetry, and confocal microscopy. We also compared the physicochemical properties of pure vesicles composed of either archaeal lipids or conventional lipids (e.g., 1,2-dipalmitoyl-sn-glycero-3-phosphocholine) with mixed vesicles composed of both lipid types. Archaeal lipids are discussed in terms of their potential use as a targeted drug delivery system based on the results of in vivo and cytotoxicity studies.

• Language: English
• Keywords: cells, lipids, membranes, stability, vesicles
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: Str. 2861-2870
• Numbering: Vol. 8, iss. 3
• PID: 20.500.12556/RUL-144325
• UDC: 577.35:582.233:615.014.6
• ISSN on article: 2470-1343
• DOI: 10.1021/acsomega.2c07406
• COBISS.SI-ID: 138234627
• Publication date in RUL: 14.02.2023

Record is a part of a journal

• Title: ACS omega
• Shortened title: ACS omega
• Publisher: American Chemical Society
• ISSN: 2470-1343
• COBISS.SI-ID: 525873945

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: liposomi, arheosomi, arheje, lipidi arhej, fizikalnokemijske lastnosti, dostavni sistemi

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0121
• Name: Biokemijska in biofizikalno-kemijska karakterizacija naravnih snovi