8-oxoguanine forms quartets with a large central cavity

Aleksič, Simon (author), Podbevšek, Peter (author), Plavec, Janez (author)

Oxidation of a guanine nucleotide in DNA yields an 8-oxoguanine nucleotide ($^{oxo}$G) and is a mutagenic event in the genome. Due to different arrangements of hydrogen-bond donors and acceptors, $^{oxo}$G can affect the secondary structure of nucleic acids. We have investigated base pairing preferences of $^{oxo}$G in the core of a tetrahelical G-quadruplex structure, adopted by analogues of d(TG$_4$T). Using spectroscopic methods, we have shown that G-quartets can be fully substituted with $^{oxo}$G nucleobases to form an $^{oxo}$G-quartet with a revamped hydrogen-bonding scheme. While an $^{oxo}$G-quartet can be incorporated into the G-quadruplex core without distorting the phosphodiester backbone, larger dimensions of the central cavity change the cation localization and exchange properties.

• Language: English
• Keywords: cations, chemical structure, G-quadruplex, monomers, noncovalent interactions
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: Str. 2390–2397
• Numbering: Vol. 61, iss. 21
• PID: 20.500.12556/RUL-144310
• UDC: 577
• ISSN on article: 1520-4995
• DOI: 10.1021/acs.biochem.2c00478
• COBISS.SI-ID: 129721091
• Publication date in RUL: 14.02.2023

Record is a part of a journal

• Title: Biochemistry
• Publisher: American Chemical Society
• ISSN: 1520-4995
• COBISS.SI-ID: 19197991

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: biokemija, DNK, g-kvadrupleksi, oksidacija

Projects

• Funder: Other - Other funder or multiple funders
• Funding programme: CERIC-ERIC

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0242
• Name: Kemija in struktura bioloških učinkovin