Antimicrobial and antibiofilm effect of commonly used disinfectants on Salmonella Infantis isolates

Bezek, Katja (author), Avberšek, Jana (author), Zorman-Rojs, Olga (author), Barlič-Maganja, Darja (author)

Salmonella enterica subsp. enterica serovar Infantis is the most prevalent serovar in broilers and broiler meat in the European Union. The aim of our study was to test the biofilm formation and antimicrobial effect of disinfectants on genetically characterized S. Infantis isolates from poultry, food, and humans. For the biofilm formation under various temperature conditions (8 °C, 20 °C, and 28 °C) and incubation times (72 h and 168 h), the crystal violet staining method was used. The evaluation of the in vitro antimicrobial effect of Ecocid® S, ethanol, and hydrogen peroxide was determined using the broth microdilution method. The antibiofilm effect of subinhibitory concentration (1/8 MIC) of disinfectants was then tested on S. Infantis 323/19 strain that had the highest biofilm formation potential. Our results showed that the biofilm formation was strain‐specific; however, it was higher at 20 °C and prolonged incubation time. Moreover, strains carrying a pESI plasmid showed higher biofilm formation potential. The antibiofilm potential of disinfectants on S. Infantis 323/19 strain at 20 °C was effective after a shorter incubation time. As shown in our study, more effective precautionary measures should be implemented to ensure biofilm prevention and removal in order to control the S. Infantis occurrence.

• Language: English
• Keywords: Salmonella Infantis, biofilm, antibiofilm, disinfectants
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 12 str.
• Numbering: Vol. 11, iss. 2, [article no.] 301
• PID: 20.500.12556/RUL-144172
• UDC: 636.09:579
• ISSN on article: 2076-2607
• DOI: 10.3390/microorganisms11020301
• COBISS.SI-ID: 140082435
• Publication date in RUL: 02.02.2023

Record is a part of a journal

• Title: Microorganisms
• Shortened title: Microorganisms
• Publisher: MDPI AG
• ISSN: 2076-2607
• COBISS.SI-ID: 523277081

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 02.02.2023

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: V4-2004
• Name: Obvladovanje okužb z bakterijo S. Infantis v rejah piščancev brojlerjev