Assessment of small cellular particles from four different natural sources and liposomes by interferometric light microscopy
ID Romolo, Anna (Author), ID Jan, Zala (Author), ID Bedina Zavec, Apolonija (Author), ID Kisovec, Matic (Author), ID Arrigler, Vesna (Author), ID Spasovski, Vesna (Author), ID Podobnik, Marjetka (Author), ID Iglič, Aleš (Author), ID Pocsfalvi, Gabriella (Author), ID Kogej, Ksenija (Author), ID Kralj-Iglič, Veronika (Author)

.pdfPDF - Presentation file, Download (1,25 MB)
MD5: 7717AC84080CDCD2FAAFFC95EA205EC1
URLURL - Source URL, Visit https://www.mdpi.com/1422-0067/23/24/15801 This link opens in a new window

Small particles in natural sources are a subject of interest for their potential role in intercellular, inter-organism, and inter-species interactions, but their harvesting and assessment present a challenge due to their small size and transient identity. We applied a recently developed interferometric light microscopy (ILM) to assess the number density and hydrodynamic radius (Rh) of isolated small cellular particles (SCPs) from blood preparations (plasma and washed erythrocytes) (B), spruce needle homogenate (S), suspension of flagellae of microalgae Tetraselmis chuii (T), conditioned culture media of microalgae Phaeodactylum tricornutum (P), and liposomes (L). The aliquots were also assessed by flow cytometry (FCM), dynamic light scattering (DLS), ultraviolet-visible spectrometry (UV-vis), and imaging by cryogenic transmission electron microscopy (cryo-TEM). In Rh, ILM showed agreement with DLS within the measurement error in 10 out of 13 samples and was the only method used here that yielded particle density. Cryo-TEM revealed that representative SCPs from Tetraselmis chuii flagella (T) did not have a globular shape, so the interpretation by Rh of the batch methods was biased. Cryo-TEM showed the presence of thin filaments in isolates from Phaeodactylum tricornutum conditioned culture media (P), which provides an explanation for the considerably larger Rh obtained by batch methods than the sizes of particles observed by cryo-TEM images. ILM proved convenient for assessment of number density and Rh of SCPs in blood preparations (e.g., plasma); therefore, its use in population and clinical studies is indicated.

Keywords:interferometric light microscopy, interferometric nanotracking analysis, nanoparticle tracking analysis, concentration of extracellular particles, size of extracellular particles, dynamic light scattering, hydrodynamic radius of extracellular particles, extracellular vesicles, liposomes, exosomes
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:ZF - Faculty of Health Sciences
FE - Faculty of Electrical Engineering
FKKT - Faculty of Chemistry and Chemical Technology
MF - Faculty of Medicine
Publication status:Published
Publication version:Version of Record
Publication date:13.12.2022
Number of pages:22 str.
Numbering:Vol. 23, no. 24, art. 15801
PID:20.500.12556/RUL-143699 This link opens in a new window
ISSN on article:1422-0067
DOI:10.3390/ijms232415801 This link opens in a new window
COBISS.SI-ID:136801795 This link opens in a new window
Publication date in RUL:09.01.2023
Copy citation
Share:Bookmark and Share

Record is a part of a journal

Title:International journal of molecular sciences
Shortened title:Int. j. mol. sci.
COBISS.SI-ID:2779162 This link opens in a new window


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:13.12.2022

Secondary language

Keywords:interferometrična svetlobna mikroskopija, interferometrična nanosledilna analiza, analiza sledenja nanodelcev, koncentracija zunajceličnih delcev, velikost zunajceličnih delcev, dinamično sipanje svetlobe, hidrodinamični radij zunajceličnih delcev, zunajcelični vezikli, liposomi, eksosomi


Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P2-0232
Name:Analiza biomedicinskih slik in signalov

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P3-0388
Name:Mehanizmi varovanja zdravja

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P1-0201
Name:Fizikalna kemija

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P1-0391
Name:Molekulske interakcije

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:J3-3066
Name:Optimizacija s trombociti in zunajceličnimi vezikli bogate avtologne krvne plazme za zdravljenje pooperativnih ran v otorinolaringologiji

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:L3-2621
Name:Mehanizmi omajanja endoproteze kolka

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:I0-0003
Name:Infrastrukturna dejavnost KI

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections: