Bisphenol A in edible tissues of rams exposed to repeated low-level dietary dose by high-performance liquid chromatography with fluorescence detection

Cerkvenik, Vesna (author), Škibin, Andrej (author), Švara, Tanja (author), Gombač, Mitja (author), Pogačnik, Milan (author), Šturm, Sabina (author)

The presented work deals with levels and distribution of bisphenol A (BPA) in the edible tissues of a large food-producing animal species. An experimental animal study included 14 young Istrian pramenka rams (Ovis aries), of which seven were exposed for 64 days to a low dietary dose of BPA at 25 µg/kg b.w./day, and seven served as a control group. Residue analysis of both aglycone and total BPA was performed in the muscle tissue, liver, kidney and fat tissue of the individual animals by means of enzymatic deconjugation (for total BPA), organic solvent extraction, molecularly imprinted polymer solid-phase extraction (MISPE) clean-up and high-performance liquid chromatography with fluorescence detection (HPLC-FLU). The analysis was optimized and validated for aglycone BPA in the fat tissue and for the total BPA in all tissues investigated. Edible tissues of the control group of rams generally remained BPA-free, while there were concentration differences between the control and treated groups for liver and kidney post last administration. The human health risk resulting from this study was assessed by the estimated dietary exposure in adults, which was < 0.1% related to the valid European Union Tolerable Daily Intake (TDI) value of 4 µg/kg b.w./day. However, it would be 58-fold higher than the newly proposed TDI value of 0.04 ng/kg b.w./day.

• Language: English
• Keywords: Bisphenol A, Total bisphenol A, Sheep, Tissues, Distribution, HPLC-fluorescence, Risk assessment
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: Str. 76078-76090
• Numbering: Vol. 29, no. 50
• PID: 20.500.12556/RUL-143570
• UDC: 636.09:615.9
• ISSN on article: 1614-7499
• DOI: 10.1007/s11356-022-21154-5
• COBISS.SI-ID: 110581763
• Publication date in RUL: 28.12.2022

Record is a part of a journal

• Title: Environmental science and pollution research
• Shortened title: Environ. sci. pollut. res. int.
• Publisher: Springer Nature
• ISSN: 1614-7499
• COBISS.SI-ID: 512607257

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0092
• Name: Zdravje živali, okolje in varna hrana