Covalently conjugated NOD2/TLR7 agonists are potent and versatile immune potentiators

Guzelj, Samo (author), Weiss, Matjaž (author), Slütter, Bram (author), Frkanec, Ruža (author), Jakopin, Žiga (author)

The success of vaccination with subunit vaccines often relies on the careful choice of adjuvants. There is great interest in developing new adjuvants that can elicit a cellular immune response. Here, we address this challenge by taking advantage of the synergistic cross-talk between two pattern recognition receptors: nucleotide-binding oligomerization-domain-containing protein 2 (NOD2) and Toll-like receptor 7 (TLR7). We designed two conjugated NOD2/TLR7 agonists, which showed potent immunostimulatory activities in human primary peripheral blood mononuclear cells and murine bone-marrow-derived dendritic cells. One of these, 4, also generated a strong antigen-specific immune response in vivo, with a Th1-polarized profile. Importantly, our study shows that novel NOD2/TLR7 agonists elicit sophisticated and fine-tuned immune responses that are inaccessible to individual NOD2 and TLR7 agonists.

• Language: English
• Keywords: immunostimulatory activity, vaccines, immune responses, pattern recognition receptors, synthesis of NOD2 agonist, Toll-like receptor 7
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: Str. 15085–15101
• Numbering: Vol. 65, iss. 22
• PID: 20.500.12556/RUL-143233
• UDC: 615.371:616-097
• ISSN on article: 1520-4804
• DOI: 10.1021/acs.jmedchem.2c00808
• COBISS.SI-ID: 128189187
• Publication date in RUL: 09.12.2022

Record is a part of a journal

• Title: Journal of medicinal chemistry
• Shortened title: J. med. chem.
• Publisher: American Chemical Society
• ISSN: 1520-4804
• COBISS.SI-ID: 512806681

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: imunostimulatorna aktivnost, imunski odziv, receptorji za prepoznavanje vzorcev, sinteza agonista NOD2, receptor TLR7, cepiva