Iskanje encimov za razgradnjo lignina v vodnih ekstraktih gliv

Žura, Lana (author), Berne, Sabina (mentor), Skočaj, Matej (co-mentor)

Lignocelulozni materiali so obnovljivi naravni vir, ki ga uporabljajo na različnih tehnoloških področjih. Težavna razgradnja lignina predstavlja oviro pri izkoriščanju ligninoceluloznega materiala. Kraljestvo gliv je bogat in neizkoriščen vir biotehnološko zanimivih encimov, med katerimi so tudi encimi, ki omogočajo razgradnjo lignina. Glavni encimi, ki sodelujejo pri razgradnji lignina so lignolitične oksidoreduktaze (lakaze, lignin peroksidaze, ter mangan peroksidaze). Cilj magistrske naloge je bil pripraviti vodne esktrakte 62 gliv nabranih v slovenskih gozdovih, ter s pomočjo testa oksidacije ABTS odkriti, ali vodni ekstrakti gliv vsebujejo encime, ki omogočajo razgradnjo lignina. Identificirali smo željene encimske aktivnosti v nekaterih vrstah gliv, ki še niso bile opisane v literaturi. Najvišje encimske aktivnosti smo določili pri glivah rodu Cantharellus, pri katerih smo podrobneje preverili tudi vpliv pH na encimsko aktivnost. Ugotovili smo, da imajo lakaze, lignin peroksidaze in mangan peroksidaze optimum delovanja večinoma v kislem in nevtralnem območju pH.

• Language: Slovenian
• Keywords: lignolitični encimi, lakaze, lignin peroksidaze, mangan peroksidaze, encimska aktivnost, ABTS, glive, vodni ekstrakti gliv, Cantharellus
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Year: 2022
• PID: 20.500.12556/RUL-142797
• COBISS.SI-ID: 131180035
• Publication date in RUL: 26.11.2022

Secondary language

• Language: English
• Title: Screening of fungal water extracts for lignin-degrading enzymes

Abstract

Lignocelullosic material is renewable natural resource, that is used in different technological areas. Difficult degradation of lignin presents an obstacle for utilization of lignocelullosic material. The kingdom of fungi is rich and unexploited source of biotechnologically interesting enzymes, including lignin-degrading enzymes. Main lignin-degrading enzymes are ligninolytic oxidoreductases (laccases, lignin peroxidases, and manganese peroxidases). The aim of this master thesis was to prepare water extracts of 62 fungi from Slovenian forests and using ABTS oxidation test discover potential lignin-degrading enzymes. In certain fungal species we identified desired enzymatic activities, that were not yet described in the literature. We determined the highest enzymatic activities in fungi from the genus Cantharellus, for which we investigated the effect of pH on enzymatic activity in more detail. We found out that laccase, lignin peroxidase and manganese peroxidase have optimum activities in the acidic and neutral pH range.

• Keywords: ligninolytic enzymes, laccase, lignin peroxidase, manganese peroxidase, enzyme activity, ABTS, fungi, fungal water extracts, Cantharellus