The influence of excipients on the viscosity of monoclonal antibody solutions

Hribar-Lee, Barbara (author)

The aggregation propensity of monoclonal antibodies can be modified by adding different cosolutes into the solution. A simple coarse-grained model in the combination with the thermodynamic perturbation theory was used to predict cluster distribution and viscosity of the solutions of IgG4 monoclonal anibody in the presence of L-Arginine Hydrochloride. The data were analysed using binding polynomial to describe the binding of cosolute (Arginine) to the antibody molecule. The results show that by binding to the antibody molecule the cosolute occupies some of the binding sites of the antibody, and in this way reduces the amount of binding sites available to other antibody molecules. The aggregation propensity of the antibody molecules is therefore reduced.

• Language: English
• Keywords: monoclonal antibody, aggregation, viscosity, cosolutes, thermodynamic perturbation theory, binding polynomial
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 5 str.
• Numbering: Vol. 366, art. 120349
• PID: 20.500.12556/RUL-142787
• UDC: 544.35:577.322
• ISSN on article: 0167-7322
• DOI: 10.1016/j.molliq.2022.120349
• COBISS.SI-ID: 121626627
• Publication date in RUL: 25.11.2022

Record is a part of a journal

• Title: Journal of molecular liquids
• Shortened title: J. mol. liq.
• Publisher: Elsevier
• ISSN: 0167-7322
• COBISS.SI-ID: 15382277

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: monoklonska protitelesa, agregacija, viskoznost, sotopljenci, termodinamska perturbacijska teorija, statistična vsota vezanja

Projects

• Funder: NIH - National Institutes of Health
• Project number: RM1GM135136