Effect of UV light and sodium hypochlorite on formation and destruction of Pseudomonas fluorescens biofilm in vitro

Sigler Zekanović, Melani (author), Begić, Gabrijela (author), Mežnarić, Silvestar (author), Jelovica Badovinac, Ivana (author), Krištof, Romana (author), Tomić Linšak, Dijana (author), Gobin, Ivana (author)

Pseudomonas fluorescens is one of the first colonizers of bacterial biofilm in water systems and a member of opportunistic premise plumbing pathogens (OPPPs). The aim of this study was to examine the effect of UV light and sodium hypochlorite on the formation and destruction of mature P. fluorescens biofilm on ceramic tiles. Planktonic bacteria or bacteria in mature biofilm were exposed to UV light (254 nm) for 5, 20 s. and to 0.4 mg/L sodium hypochlorite for 1 min. Mature biofilm was also exposed to increased concentration of sodium hypochlorite of 2 mg/L for 0.5, 1 and 2 h and combined with UV. Prolonged action of sodium hypochlorite and an increase in its concentration in combination with UV gave the best results in the inhibition of biofilm formation after the pretreatment and destruction of mature biofilm. The effect of hyperchlorination in combination with UV radiation shows better results after a long exposure time, although even after 120 min there was no completely destroyed biofilm. Furthermore, the mechanism of the effect of combined methods should be explored as well as the importance of mechanical cleaning that is crucial in combating bacterial biofilm in swimming pools.

• Language: English
• Keywords: biofilm, disinfection, opportunistic pathogens, Pseudomonas fluorescens, UV light
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: ZF - Faculty of Health Sciences
• Publication status: Published
• Publication version: Version of Record
• Publication date: 01.01.2022
• Year: 2022
• Number of pages: 15 str.
• Numbering: Vol. 10, no. 10, art. 1901
• PID: 20.500.12556/RUL-142784
• UDC: 614:628.166
• ISSN on article: 2227-9717
• COBISS.SI-ID: 130886147
• Publication date in RUL: 25.11.2022

Record is a part of a journal

• Title: Processes
• Shortened title: Processes
• Publisher: MDPI
• ISSN: 2227-9717
• COBISS.SI-ID: 523353113

Licences

• License: CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
• Link: http://creativecommons.org/licenses/by-nc/4.0/
• Description: A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.

Secondary language

• Language: Undetermined
• Keywords: biofilm, dezinfekcija, oportunistični patogeni, Pseudomonas fluorescens, UV svetloba