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Helicobacter colisuis sp. nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus)
ID Gruntar, Igor (Author), ID Kostanjšek, Rok (Author), ID Pirš, Tina (Author), ID Papić, Bojan (Author)

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Abstract
Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp. in 2015. The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates. Morphologically, the bacterial cells were spirilli-shaped and highly motile, 1–2 µm long and ≤0.5 µm wide, Gram-negative, oxidase-positive and catalase-positive. They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum. Three strains (11154-15$^T$, 14348–15 and 16470–15) underwent whole-genome sequencing. Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis. Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %). The core genome-based phylogeny confirmed that the new strains form a distinct clade most closely related to H. canadensis. The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel Helicobacter species for which the name Helicobacter colisuis sp. nov. is suggested, with strain 11154-15$^T$ (= DSM 113688$^T$ = CCUG 76053$^T$) as the type strain.

Language:English
Keywords:Helicobacter, polymerase chain reaction, Sus scrofa, caecum, domestic pig, Sus scrofa domesticus, novel species
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:VF - Veterinary Faculty
BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Year:2022
Number of pages:7 str.
Numbering:Vol. 72, iss. 11, art. 005600
PID:20.500.12556/RUL-142718 This link opens in a new window
UDC:636.09:579
ISSN on article:1466-5034
DOI:10.1099/ijsem.0.005600 This link opens in a new window
COBISS.SI-ID:130261763 This link opens in a new window
Publication date in RUL:22.11.2022
Views:349
Downloads:188
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Record is a part of a journal

Title:International journal of systematic and evolutionary microbiology
Shortened title:Int. j. syst. evol. microbiol.
Publisher:Microbiology Society
ISSN:1466-5034
COBISS.SI-ID:521767193 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P4-0092
Name:Zdravje živali, okolje in varna hrana

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