Color variation and secondary metabolites’ footprint in a taxonomic complex of Phyteuma sp. (Campanulaceae)

Grohar, Mariana Cecilia (author), Medič, Aljaž (author), Ivančič, Tea (author), Veberič, Robert (author), Jogan, Jernej (author)

In the genus Phyteuma, the taxonomic delimitation of some species is difficult since a high variability of morphological traits, such as flower color, is present, probably due to high levels of hybridization. Historic descriptions and the morphological traits used in the taxonomic keys are sometimes unclear and lead to misinterpretations. Here, a detailed analysis of flower color variability in different populations of sympatric P. spicatum, P. ovatum, and P. persicifolium constitutes a new approach to clarifying the taxonomic statuses. The numeric analysis of color, providing colorimetric variables, together with the detailed description of the metabolic profiles of populations with different flower colors, constitute a unique chemical fingerprint that identifies species and subspecies with clear markers. This study is the most complete metabolic research on genus Phyteuma, since we identified and quantified 44 phenolic compounds using HPLC-MS, comprising 14 phenolic acids, 23 flavonols and flavones, and, for the first time in the genus, 7 anthocyanins involved in flower color variability. This approach contributes to clarifying the differences between species, which is particularly relevant in taxonomic complexes such as the present, where morphology fails to clearly differentiate taxa at specific and intraspecific levels.

• Language: English
• Keywords: Phyteuma, phenolic compounds, anthocyanins, phenolic acids, flavonols, HPLC-MS, identification, Slovenia
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 15 str.
• Numbering: Vol. 11, iss. 21, art. 2894
• PID: 20.500.12556/RUL-142495
• UDC: 634.11/.12
• ISSN on article: 2223-7747
• DOI: 10.3390/plants11212894
• COBISS.SI-ID: 128980483
• Publication date in RUL: 11.11.2022

Record is a part of a journal

• Title: Plants
• Shortened title: Plants
• Publisher: MDPI
• ISSN: 2223-7747
• COBISS.SI-ID: 523345433

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: fenolne spojine, fenolne kisline, antocianini, flavonoli, identifikacija, sekundarni metaboliti, taksonomija

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0013
• Name: Hortikultura