Domestication is a process of artificial selection and breeding of wild species to obtain new variants. A key feature of domestication is adaptation to the man-made environment. The aim of this study was to phenotypically characterise strains of Saccharomyces and Hanseniaspora species and to determine whether a wild or manmade environment as the origin of isolation had an effect on their phenotype. Phenotyping was performed on solid media using a Cartesian manipulator with realtime colony growth measurements. Using these data, we determined the relative growth rate on solid media with the addition of various growth inhibitors, such as ethanol, acetic acid, lactic acid, ethylphenol, ethyl guaiacol, copper, sulfite and maltose. We chose these factors because the yeasts are exposed to such conditions during alcoholic fermentation or spraying in the vineyard. Domesticated strains were represented by strains isolated from vineyards, olive trees and fruits, while wild strains were isolated from forests. Domesticated Saccharomyces strains exhibited increased tolerance to copper and sulfite and were resistant to higher concentrations of ethanol, acetic acid, lactic acid, and ethyl guaiacol. We detected increased tolerance to copper, sulfite, and ethylguaiacol in Hanseniaspora strains. The Hanseniaspora strains did not grow on other media. We also isolated wild strains of the Saccharomyces genus from forests in the Koroška, Gorenjska, and Primorska regions, while strains of the Hanseniaspora genus were isolated only in the Koroška region. The main source of isolates of both genera was oak bark. The strains of the genus Saccharomyces isolated from the forests belonged to the species S. paradoxus, S. cerevisiae, S. jurei and S. kudriavzevii. Of the genus Hanseniaspora, only the species H. osmophila and H. uvarum were isolated from the forest.
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