Disinfecting action of gaseous ozone on OXA-48-producing Klebsiella pneumoniae biofilm in vitro

Piletić, Kaća (author), Kovač, Bruno (author), Perčić, Marko (author), Žigon, Jure (author), Broznic, Dalibor (author), Karleuša, Ljerka (author), Lučić Blagojević, Sanja (author), Oder, Martina (author), Gobin, Ivana (author)

Klebsiella pneumoniae is an emerging multidrug-resistant pathogen that can contaminate hospital surfaces in the form of a biofilm which is hard to remove with standard disinfectants. Because of biofilm resistance to conservative disinfectants, the application of new disinfection technologies is becoming more frequent. Ozone gas has antimicrobial activity but there is lack of data on its action against K. pneumoniae biofilm. The aim of this study was to investigate the effects and mechanisms of action of gaseous ozone on the OXA-48-procuding K. pneumoniae biofilm. A 24 h biofilm of K. pneumoniae formed on ceramic tiles was subsequently exposed to different concentrations of ozone during one and two hours to determine the optimal ozone concentration. Afterwards, the total bacteria count, total biomass and oxidative stress levels were monitored. A total of 25 ppm of gaseous ozone was determined to be optimal ozone concentration and caused reduction in total bacteria number in all strains of K. pneumoniae for 2.0 log10 CFU/cm2, followed by reduction in total biomass up to 88.15%. Reactive oxygen species levels significantly increased after the ozone treatment at 182% for the representative K. pneumoniae NCTC 13442 strain. Ozone gas in the concentration of 25 ppm caused significant biofilm reduction but did not completely eradicate the K. pneumoniae biofilm formed on ceramics. In conclusion, ozone gas has great potential to be used as an additional hygiene measure in joint combat against biofilm in hospital environments.

• Language: English
• Keywords: biofilm, gaseous disinfection, hospital-acquired infections, multidrug-resistant microorganisms, OXA-48 disinfection, ozone
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Publication date: 19.05.2022
• Year: 2022
• Number of pages: 18 str.
• Numbering: Vol. 19, iss. 10
• PID: 20.500.12556/RUL-142066
• UDC: 614
• ISSN on article: 1660-4601
• DOI: 10.3390/ijerph19106177
• COBISS.SI-ID: 108768003
• Publication date in RUL: 19.10.2022

Record is a part of a journal

• Title: International journal of environmental research and public health
• Shortened title: Int. j. environ. res. public health
• Publisher: MDPI
• ISSN: 1660-4601
• COBISS.SI-ID: 1818965

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: Klebsiella pneumoniae, biofilm, plinska dezinfekcija, bolnišnične okužbe, mikroorganizmi, rezistenca, zdravila, OXA-48 dezinfekcija

Projects

• Funder: Other - Other funder or multiple funders
• Project number: 18-171
• Name: UNIRI-biomed 18-171