GC–MS analysis of A Helichrysum italicum hydrosol : sensitivity, repeatability and reliability of solvent extraction versus direct hydrosol analysis

Kokalj, Meta (author), Kočevar Glavač, Nina (author)

Hydrosols have been increasingly used in the food industries as drinks and as ingredients for seasoning and flavoring. Research has shown they have antimicrobial, antioxidative and anti-inflammatory effects and therefore have potential as food preservative agents and sanitizing agents for tools used in the food industry. The composition analysis of hydrosols is commonly conducted using extraction with organic solvents. However, some hydrophilic compounds are lost with this procedure, leading to issues in identifying native composition and potential adulteration. In this paper, different methods for hydrosol analysis are studied. The methods differ in sample preparation, number of detected compounds, repeatability and reliability. Direct hydrosol analysis and 1:1 extraction with organic solvent were determined to be the most appropriate in our experiments. However, the method implementation depends on the application. Direct hydrosol analysis is simple and does not change ratios among compounds; its drawback is poor repeatability. Using 1:1 extraction has good repeatability; however, some hydrophilic compounds are lost, and adulterations with cosolvents and the addition of solubilizers cannot be discovered.

• Language: English
• Keywords: hydrosol, GC-MS analysis, Helichrysum italicum, volatile compounds
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 11 str.
• Numbering: Vol. 12, iss. 19, art. 10040
• PID: 20.500.12556/RUL-141875
• UDC: 542.48:665.5
• ISSN on article: 2076-3417
• DOI: 10.3390/app121910040
• COBISS.SI-ID: 124905731
• Publication date in RUL: 10.10.2022

Record is a part of a journal

• Title: Applied sciences
• Shortened title: Appl. sci.
• Publisher: MDPI
• ISSN: 2076-3417
• COBISS.SI-ID: 522979353

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: hidrosoli, GC-MS analiza, Helichrysum italicum, hlapne spojine, eterična olja

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0208-2015
• Name: Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin