The role of endothelin B receptor in bone modelling during orthodontic tooth movement : a study on ET B knockout rats

Disha Ibrahimi, Saranda (author), Furlani, Borut (author), Drevenšek, Gorazd (author), Plut, Alja (author), Yanagisawa, M (author), Hudoklin, Samo (author), Prodan Žitnik, Irena (author), Marc, Janja (author), Drevenšek, Martina (author)

The endothelin system has an important role in bone modelling during orthodontic tooth movement (OTM); however, little is known about the involvement of endothelin B receptors (ETB) in this process. The aim of this study was to evaluate the role of ETB in bone modelling during OTM using ETB knockout rats (ETB-KO). Thirty-two male rats were divided into 4 groups (n = 8 per group): the ETB-KO appliance group, ETB-KO control group, wild type (ETB-WT) appliance group, and ETB-WT control group. The appliance consisted of a super-elastic closed-coil spring placed between the first and second left maxillary molar and the incisors. Tooth movement was measured on days 0 and 35, and maxillary alveolar bone volume, osteoblast, and osteoclast volume were determined histomorphometrically on day 35 of OTM. Next, we determined the serum endothelin 1 (ET-1) level and gene expression levels of the osteoclast activity marker cathepsin K and osteoblast activity markers osteocalcin and dentin matrix acidic phosphoprotein 1 (DMP1) on day 35. The ETB-KO appliance group showed significantly lower osteoblast activity, diminished alveolar bone volume and less OTM than the ETB-WT appliance group. Our results showed that ETB is involved in bone modelling in the late stage of OTM.

• Language: English
• Keywords: orthodontic tooth movement, endothelin system, bone modelling
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy; MF - Faculty of Medicine
• Publication version: Version of Record
• Publication date: 01.01.2020
• Year: 2020
• Number of pages: 11 str.
• Numbering: art. 14226, Vol. 10
• PID: 20.500.12556/RUL-141299
• UDC: 616.31
• ISSN on article: 2045-2322
• DOI: 10.1038/s41598-020-71159-8
• COBISS.SI-ID: 27363587
• Publication date in RUL: 28.09.2022

Record is a part of a journal

• Title: Scientific reports
• Shortened title: Sci. rep.
• Publisher: Nature Publishing Group
• ISSN: 2045-2322
• COBISS.SI-ID: 18727432

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: ortodontsko gibanje zob, endotelinski sistem, modeliranje kosti

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0293-2020
• Name: Parodontalna medicina