Introduction: Many people use thermal pools for different purposes. However, with the greater frequency of the users, it is necessary to provide pool water in accordance with the regulations for all users. To ensure that, disinfection is used. With disinfection, we prevent the transfer of dispersal of microorganisms. Besides that, disinfection agent also reacts with the substances in the pool water, brought in water by the people. That reaction produces different disinfection by-products. Purpose: The purpose of this Master thesis is to research the area of origin of the side products present at the disinfection of pool water with the simultaneous use of herbal extracts. Methods: First, we looked at the existing literature on which we based our research. Based on the extracted data we then prepared a simulation of an herbal bath. The heater in the bath provided a steady temperature of 35 °C. The samples were heated in the bath in erlenmeyers and vials. All the samples were being taken out of the herbal bath in predetermined time intervals and were analyzed with the SPME method (samples of the gas phase) or with the liquid/liquid extraction (samples of the liquid phase). The analysis was being taken in both seawater and distilled water. After that, the samples from the liquid phase had to be extracted with dichloromethane, vaporized and then analysed on GS-MS. The samples from the gas phase required no previous preparation. The analysis was being carried out on a gas chromatograph and was followed by an identification of compounds that were made during the process of chlorination. Results: With the experimental work, we proved the occurrence of side products that is produced in the water with the simultaneous use of the disinfection agent and herbal extract, in our case limonene. In all of our samples, taken during the different time periods, we identified 18 different compounds. Disinfection by-products were detected in liquid and gas phase, the liquid phase had more detected compounds. Disinfection by-products were identified in both sea and distilled water. During the chlorination, we identified different oxides, primary, secondary and tertiary products of adding chlorine and primary products of bromination in seawater. Discussion and conclusion: In the samples that were exposed to the addition of chlorine for a longer period, we identified more different compounds than in the samples that were exposed for a shorter period. In the samples that were being taken at a higher temperature, we proved the occurrence of oxides, which were not detected in samples taken at room temperature. In the samples of seawater, we identified the compounds with bromine that were not present in distilled water. In some samples, we identified the emergence of trihalometane.
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