Reactive oxygen species (ROS), located in chloroplasts, play an important role in the plant immune response to stress. To examine the role of chloroplastic ROS in potato plants, we reduced the amount of ROS in the chloroplasts of potato plants by expressing the chloroplast-targeted protein flavodoxin by transient transformation. We used transgenic potato plants pt-roGFP2 NahG-Rywal, which enable the monitoring of the redox state in chloroplasts due to the redox-sensitive protein GFP (roGFP) located in chloroplasts. According to the results obtained, we can conclude that flavodoxin has an influence on the redox state in the chloroplasts of pt-roGFP2 NahG-Rywal potato plants, as plants with the inserted gene for flavodoxin had, on average, more reduced redox state in the chloroplasts three days after agroinfiltration. On the other hand, six days after agroinfiltration, there were no visible differences in the redox state between plants with flavodoxin and control plants. After six days, the redox state in potato chloroplasts was more oxidized than three days after agroinfiltration, which can be attributed to other factors, such as the aging of the plant or senescence. In addition, we found that compared to potato, tobacco is a more suitable plant for agroinfiltration if we want to obtain samples with a relatively high expression of flavodoxin.
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