Sistem za nadzor temperature pri poizkusih na ravninskih lipidnih dvoslojih

JAGODIČ, TILEN (author), Kramar, Peter (mentor)

Ravninski lipidni dvosloji so izredno preprost, a dober model celične membrane za preučevanje izpostavitve električnemu polju. Zaradi narave lipidnih molekul in njihove formacije v različne kristalne strukture, ki so odvisne od temperature, ima pri poizkusih na ravninskih lipidnih dvoslojih pomemben vpliv tudi temperatura. Za spreminjanje temperature smo uporabili regulator temperature Thermocube, ki mu lahko temperaturo nastavimo z uporabo serijske komunikacije RS-232. Thermocube ima različne možnosti delovanja in s tem tudi načine komunikacije. Zato ta poteka v večih korakih. Za krmiljenje je uporabljen miniračunalnik Raspberry Pi, na katerem je napisan preprost program za serijsko komunikacijo med regulatorjem in termočlenom, ki ima nalogo termometra. V diplomski nalogi je opisan potek razvoja sistema za nadzor temperature pri ravninskih lipidnih dvoslojih. Pri tem so opisani komunikacijski protokoli, razvoj programov in delovanje regulatorja temperature Thermocube ter termočlena.

• Language: Slovenian
• Keywords: Thermocube, RS-232, lipidi, Raspberry Pi, temperatura, termočlen
• Work type: Bachelor thesis/paper
• Organization: FE - Faculty of Electrical Engineering
• Year: 2022
• PID: 20.500.12556/RUL-139997
• COBISS.SI-ID: 120859139
• Publication date in RUL: 09.09.2022

Secondary language

• Language: English

Abstract

Planar lipid bilayers are an extremely simple but good cell membrane model for studying electric field exposure. Due to the nature of lipid molecules and their formation into different crystal structures, temperature has an important influence on experiments with planar lipid bilayers. A Thermocube chiller is used to regulate the temperature and uses the RS-232 serial protocol for communication. The thermoelectric recirculating chiller has various operating options and thus also communication methods. A Raspberry Pi minicomputer is used to communicate with chiller and thermocouple as temperature sensor. For that purpose we wrote a simple program for serial communication with the chiller and the thermocouple. The thesis describes the course of development of the temperature control system for planar lipid bilayers. Therefore, communication protocols, program development and operation of the Thermocube chiller and thermocouple are described.

• Keywords: Thermocube, RS-232, lipids, Raspberry Pi, temperature, thermocouple