Models of electroporation and the associated transmembrane molecular transport should be revisited
ID Scuderi, Maria (Author), ID Dermol-Černe, Janja (Author), ID da Silva, Clarissa Amaral (Author), ID Muralidharan, Aswin (Author), ID Boukany, Pouyan E. (Author), ID Rems, Lea (Author)

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Electroporation has become a powerful tool for nonviral delivery of various biomolecules such as nucleic acids, proteins, and chemotherapeutic drugs to virtually any living cell by exposing the cell membrane to an intense pulsed electric field. Different multiphysics and multiscale models have been developed to describe the phenomenon of electroporation and predict molecular transport through the electroporated membrane. In this paper, we critically examine the existing mechanistic, single-cell models which allow spatially and temporally resolved numerical simulations of electroporation-induced transmembrane transport of small molecules by confronting them with different experimental measurements. Furthermore, we assess whether any of the proposed models is universal enough to describe the associated transmembrane transport in general for all the different pulse parameters and small molecules used in electroporation applications. We show that none of the tested models can be universally applied to the full range of experimental measurements. Even more importantly, we show that none of the models has been compared to sufficient amount of experimental data to confirm the model validity. Finally, we provide guidelines and recommendations on how to design and report experiments that can be used to validate an electroporation model and how to improve the development of mechanistic models.

Keywords:electropermeabilization, mathematical modeling, electrodiffusion, molecular transport, multiphysics, multiscale
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:FE - Faculty of Electrical Engineering
Publication status:Published
Publication version:Version of Record
Number of pages:13 str.
Numbering:Vol. 147, art. 108216
PID:20.500.12556/RUL-139608 This link opens in a new window
ISSN on article:1567-5394
DOI:10.1016/j.bioelechem.2022.108216 This link opens in a new window
COBISS.SI-ID:117683971 This link opens in a new window
Publication date in RUL:06.09.2022
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COBISS.SI-ID:2502484 This link opens in a new window


License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

Keywords:elektropermeabilizacija, matematično modeliranje, elektrodifuzija, transport molekul, multifizika, modeliranje na več ravneh


Funder:ARRS - Slovenian Research Agency
Project number:J2-2503
Name:Vpliv visokonapetostnih električnih pulzov na membranske proteine pri elektroporaciji

Funder:EC - European Commission
Funding programme:H2020
Project number:893077
Name:Controlling the susceptibility of biological cells to pulsed electric field treatment by using ion channel modulators

Funder:NWO - Netherlands Organisation for Scientific Research
Funding programme:ENW-M-2
Project number:OCENW.M20.308

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