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A novel approach using growth curve analysis to distinguish between antimicrobial and anti-biofilm activities against Salmonella
ID Sterniša, Meta (Author), ID Sabotič, Jerica (Author), ID Klančnik, Anja (Author)

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Abstract
Salmonella spp. are a commonly identified cause of outbreaks of food-borne diseases. Despite much research, there remains the need to find new antimicrobial and anti-biofilm agents against Salmonella. For this, it is necessary to distinguish between these two aspects. Agents that influence biofilm formation should not affect bacterial growth, to thus avoid further promotion of the development of resistance. In this study, we present the use of growth curves of Salmonella Infantis to simultaneously determine antimicrobial and anti-biofilm activities, for the screening for anti-Salmonella activities of 42 aqueous fungal extracts. The extract from Pseudohydnum gelatinosum showed good antimicrobial activity, and that from Pleurotus ostreatus showed good anti-biofilm activity. In extracts from Infundibulicybe geotropa and Infundibulicybe gibba, both activities were determined after fractionation. The antimicrobial activity was associated with protein-rich fractions and mediated by L-amino acid oxidase activity. The fractionation did not allow determination of the anti-biofilm active fraction, so further studies are needed to define these compounds. Growth curve analysis of S. Infantis is shown here to provide a fast and simple approach to distinguish between antimicrobial and anti-biofilm activities in a high-throughput setting, such that it can be easily implemented in screening and further bioassay-based purification of novel alternatives to antibiotics.

Language:English
Keywords:Salmonella, antimicrobial, anti-biofilm, fungal extracts, biofilm, growth curve
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Year:2022
Number of pages:11 str.
Numbering:Vol. 364, art. 109520
PID:20.500.12556/RUL-139060 This link opens in a new window
UDC:579.67:579.24
ISSN on article:0168-1605
DOI:10.1016/j.ijfoodmicro.2021.109520 This link opens in a new window
COBISS.SI-ID:92026115 This link opens in a new window
Publication date in RUL:29.08.2022
Views:633
Downloads:129
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Record is a part of a journal

Title:International journal of food microbiology
Shortened title:Int. j. food microbiol.
Publisher:Elsevier
ISSN:0168-1605
COBISS.SI-ID:6316551 This link opens in a new window

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

Language:Slovenian
Keywords:Salmonella, protimikrobne snovi, biofilm, ekstrakti gliv

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P4-0116
Name:Mikrobiologija in biotehnologija živil in okolja

Funder:ARRS - Slovenian Research Agency
Project number:J4-1771
Name:Nove protimikrobne strategije preprečevanja tvorbe biofilma z uporabo lektinov, ki inhibirajo bakterijsko adhezijo

Funder:ARRS - Slovenian Research Agency
Project number:J4-9299
Name:Mehanizmi adhezije bakterij Campylobacter kot tarča za zmanjšanje antibiotske odpornosti

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