Določitev hlapnih komponent v medu

Van Elteren, Jan (author), Kočar, Drago (mentor)

V diplomski nalogi sem določal hlapne komponente v cvetličnem in gozdnem medu. Uporabljal sem HS-SPME-GC-MS. Preveril sem ponovljivost in linearnost metode, ki sta bili zadovoljivi. Določil sem 40 najbolj zastopanih hlapnih spojin v vsaki od vrst medu, ki so ustrezale določenim izbirnim kriterijem. Razdelil sem jih na skupne in na specifične spojine, prisotne le v eni od vrst medu. Skupnih je bilo 26, prevladovali so estri, terpeni in derivati benzena. V obeh vrstah medu so največji odziv dale skupne spojine hotrienol, p-(1-propenil)toluen, metil nonanoat, metil-(Z)-9-oktadecenoat in metil oktanoat. Od specifičnih so bili v cvetličnem medu najbolj zastopani 1,2-dihidronaftalen, benzil nitril in 1-metoksi-4-metilbenzen. V gozdnem so največji odziv imeli terpinen-4-ol, α-terpineol in metil (E)-2-nonenoat. Od teh se 1,2-dihidronaftalen in benzil nitril nista pojavila v niti enem od vzorcev gozdnega medu. Od naštetih spojin, specifičnih za gozdni med, se ni nobena pojavila v niti enem od vzorcev cvetličnega medu. Te spojine bi bile lahko uporabne kot določitveni markerji za slovenski cvetlični in gozdni med.

• Language: Slovenian
• Keywords: cvetlični med, gozdni med, hlapne organske spojine, HS-SPME-GC-MS
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2022
• PID: 20.500.12556/RUL-138922
• COBISS.SI-ID: 120795395
• Publication date in RUL: 25.08.2022

VAN ELTEREN, Jan, 2022, Določitev hlapnih komponent v medu [online]. Bachelor’s thesis. [Accessed 27 March 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=138922

Secondary language

• Language: English
• Title: Determination of volatile compounds in honey

Abstract

In this thesis I determined the volatile components of mixed floral and forest honey. I used HS-SPME-GC-MS. I examined the reproducibility and linearity of the method, which were satisfactory. I identified the 40 most represented volatile compounds in both types of honey that met certain selection criteria. I divided them into common and into specific compounds, present in only one type of honey. There were 26 common compounds, predominantly esters, terpenes and benzene derivatives. In both honeys, the common compounds hotrienol, p-(1-propenyl) toluene, methyl nonanoate, methyl-(Z)-9-octadecenoate and methyl octanoate were the most abundant. Of the specific components, 1,2-dihydronaphthalene, benzyl nitrile and 1-methoxy-4-methylbenzene had the highest signal in floral honey, while terpinene-4-ol, α-terpineol and methyl (E)-2-nonenoate appeared in forest honey with the highest signal. Of these, 1,2-dihydronaphthalene and benzyl nitrile were not present in any of the forest honeys. None of the listed forest-specific volatiles appeared in any of the floral honey samples. The stated compounds could be used as identification markers for Slovenian mixed floral and forest honey.

• Keywords: floral honey, forest honey, volatile organic compounds, HS-SPME-GC-MS