In my thesis, I developed and partially validated an HPLC method for the
determination of five B vitamins in food supplements. The work included preparation of
appropriate standard solutions, optimization of the analytical method conditions,
preparation of calibration curves, partial validation of the method, sample preparation and
analysis. I have managed to optimize the method to the extent that it can be used to
qualitatively and quantitatively determine the content of five B vitamins in simple watersoluble samples. The partial validation included determination of linearity, repeatability,
limit of detection and limit of quantification. The method can be applied to the analysis
of samples containing niacin (vitamin B3), pantothenic acid (vitamin B5), pyridoxine
(vitamin B6), biotin (vitamin B7), and cyanocobalamin (vitamin B12). I have tested the
method by analysing two samples. I determined the vitamins in Siti vitamin instant drink
with orange flavour and in CBD water-soluble drops with vitamin B complex.
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