Protiglivna aktivnost funkcionalizirane folije z izvlečkom rjave alge Padina pavonica

Pecik, Pia (author), Smole-Možina, Sonja (mentor)

Globalizacija in ozaveščenost potrošnikov pospešeno spodbujata industrijo konzerviranja in pakiranja živil k uporabi okolju in zdravju prijaznih naravnih virov protimikrobnih materialov in dodatkov. Eden izmed uspešnih biorazgradljivih materialov so folije na osnovi polimlečne kisline. Ker folije same nimajo protimikrobnega učinka, jim za izboljšanje te funkcije dodajajo različne protimikrobne in antioksidativne polimere. Hitozan je eden izmed najpogosteje uporabljenih polimernih dodatkov zaradi dobrih mehanskih ter fizikalno-kemijskih lastnosti. Za izboljšanje protimikrobne aktivnosti mu še dodajajo bioaktivne snovi, kot so eterična olja ali izvlečki alg in rastlin. Z diplomsko nalogo smo preverili protiglivno aktivnost različnih oblik hitozanskega premaza (primarni in kvarterni) z vgrajenim dodatkom izvlečka rjave alge Padina pavonica proti glivi Aspergillus flavus. Pri testiranju smo ugotavljali protimikrobno in antioksidativno aktivnost štirih različnih formulacij folij z metodo ISO 22196. Z eksperimentalnim delom smo potrdili boljši protimikrobni učinek, če je bil izvleček alge kapsuliran s kvarterno obliko hitozana (69,91 in 69,12 %). Vzorci, kapsulirani s primarno obliko hitozana, so dosegli 45,25 in 40,34 % aktivnost. Pri antioksidativnem učinku izstopa le formulacija, kjer je kvarterni hitozan uporabljen tako pri osnovnem premazu, kot pri kapsulaciji izvlečka (25,22 % SA). Ostale formulacije so imele statistično neznačilne antioksidativne lastnosti (16,48 %, 14,05 % in 13,38 % SA).

• Language: Slovenian
• Keywords: konzerviranje živil, pakiranje živil, aktivno pakiranje, protimikrobno delovanje, protimikrobne snovi, Padina pavonica, funkcionalizirane folije, PLA folije, Aspergillus flavus
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: BF - Biotechnical Faculty
• Place of publishing: Ljubljana
• Publisher: [P. Pecik]
• Year: 2022
• PID: 20.500.12556/RUL-138858
• UDC: 621.798:579.24:664.0
• COBISS.SI-ID: 119286275
• Publication date in RUL: 24.08.2022

PECIK, Pia, 2022, Protiglivna aktivnost funkcionalizirane folije z izvlečkom rjave alge Padina pavonica [online]. Bachelor’s thesis. Ljubljana : P. Pecik. [Accessed 20 March 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=138858

Secondary language

• Language: English
• Title: Antifungal activity of functionalized foil with brown algae Padina pavonica extract

Abstract

Globalization and consumer awareness are rapidly pushing the food preservation and packaging industry towards the environment and health less harmful natural sources of antimicrobial materials and additives. One of the most successful biodegradable material is based on polylactic acid foil. The foil itself does not have any antimicrobial effect, therefore various antimicrobial and antioxidant polymers are added for functionality improvement. Chitosan is one of the extensively used polymer additives due to its effective mechanical and physicochemical properties. To improve its antimicrobial activity, bioactive substances such as essential oils or extracts of algae and plants are added. Within our work we tested antifungal activity against the Aspergillus flavus with various forms of chitosan coatings (with primary and quaternary chitosan formulations and encapsulated extract of brown algae Padina pavonica. Throughout the experiment, we determined antimicrobial and antioxidant activity of four different foil coating formulations by ISO 22196 method. Experimental results revealed better antimicrobial and antioxidant effect in samples with quaternary chitosan used for algae extract encapsulation (69,91 % and 69,12 % activity). Formulations with primary chitosan for encapsulation expressed 45,25 and 40,34 % activity, recpectively. Antioxidant test showed significant difference only in formulation where quaternary chitosan was used in base coating as well as for extract encapsulation (25,22 % SA). Other formulations showed slight antioxidant activity, which was not statisticaly significant (16,48 %, 14,05 % and 13,38 % SA, respectively).

• Keywords: food preservation, food packaging, active packaging, antimicrobial activity, antimicrobial substances, Padina pavonica, functionalized foils, PLA foils, Aspergillus flavus