Surface plasmon resonance approach to study drug interactions with SARS-CoV-2 RNA-dependent RNA polymerase highlights treatment potential of suramin

Mravinec, Martina (author), Bajc, Gregor (author), Butala, Matej (author)

The SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) is essential for virus replication, therefore it is a promising drug target. Here we present a surface plasmon resonance approach to study the interaction of RdRp with drugs in real time. We monitored the effect of favipiravir, ribavirin, sofosbuvir triphosphate PSI-7409 and suramin on RdRp binding to RNA immobilized on the chip. Suramin precluded interaction of RdRp with RNA and even displaced RdRp from RNA.

• Language: English
• Keywords: SARS-CoV-2, RNA-dependent RNA polymerase, surface plasmon resonance, suramin
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 4 str.
• Numbering: Vol. 298, art. 114283
• PID: 20.500.12556/RUL-138839
• UDC: 577.2
• ISSN on article: 0166-0934
• DOI: 10.1016/j.jviromet.2021.114283
• COBISS.SI-ID: 76436739
• Publication date in RUL: 23.08.2022

Record is a part of a journal

• Title: Journal of virological methods
• Shortened title: J. virol. methods
• Publisher: Elsevier
• ISSN: 0166-0934
• COBISS.SI-ID: 25810432

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: površinska plazmonska resonanca, suramin, SARS-CoV-2, covid-19

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0207
• Name: Toksini in biomembrane

• Funder: ARRS - Slovenian Research Agency
• Project number: J4-1778
• Name: Uporaba malega proteina bakteriofaga v boju proti razvoju odpornosti proti antibiotikom pri bakteriji Staphylococcus aureus