Cell death due to electroporation : a review

Batista Napotnik, Tina (author), Polajžer, Tamara (author), Miklavčič, Damijan (author)

Exposure of cells to high voltage electric pulses increases transiently membrane permeability through membrane electroporation. Electroporation can be reversible and is used in gene transfer and enhanced drug delivery but can also lead to cell death. Electroporation resulting in cell death (termed as irreversible electroporation) has been successfully used as a new non-thermal ablation method of soft tissue such as tumours or arrhythmogenic heart tissue. Even though the mechanisms of cell death can influence the outcome of electroporation-based treatments due to use of different electric pulse parameters and conditions, these are not elucidated yet. We review the mechanisms of cell death after electroporation reported in literature, cell injuries that may lead to cell death after electroporation and membrane repair mechanisms involved. The knowledge of membrane repair and cell death mechanisms after cell exposure to electric pulses, targets of electric field in cells need to be identified to optimize existing and develop of new electroporation-based techniques used in medicine, biotechnology, and food technology.

• Language: English
• Keywords: apoptosis, cell death, cell injury, electroporation, membrane repair, necroptosis, necrosis, pyroptosis
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 18 str.
• Numbering: Vol. 141, art. 107871
• PID: 20.500.12556/RUL-138669
• UDC: 577:602.621
• ISSN on article: 1567-5394
• DOI: 10.1016/j.bioelechem.2021.107871
• COBISS.SI-ID: 69133827
• Publication date in RUL: 08.08.2022

Record is a part of a journal

• Title: Bioelectrochemistry
• Publisher: Elsevier
• ISSN: 1567-5394
• COBISS.SI-ID: 2502484

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: apoptoza, celična smrt, poškodba celice, elektroporacija, celjenje membrane, nekroptoza, nekroza, piroptoza