Loss of H3K27me3 expression in canine nerve sheath tumors

Tekavec, Kristina (author), Švara, Tanja (author), Knific, Tanja (author), Mlakar, Jernej (author), Gombač, Mitja (author), Cantile, Carlo (author)

Nerve sheath tumors (NSTs) are characterized by neoplastic proliferation of Schwann cells, perineurial cells, endoneurial and/or epineurial fibroblasts. Diagnosis of NST is often challenging, particularly in distinguishing malignant NST (MNST) from other soft tissue sarcomas, or sometimes between low-grade MNST and benign NST. Recent studies in human pathology have demonstrated loss of trimethylation at lysine 27 of histone 3 (H3K27me3) in a subset of MNSTs using immunohistochemistry. Loss of H3K27me3 expression is rare in other high-grade sarcomas and also appears to be useful in distinguishing benign and low-grade MNSTs from high-grade subsets. In our retrospective study, we performed H3K27me3 immunohistochemistry in 68 canine tumors previously diagnosed as NST. We detected loss of H3K27me3 expression in 25% (n = 17) of all canine NST, including one neurofibroma, whereas 49% (n = 33) of tumors had mosaic loss of expression and 26% (n = 18) retained expression. No statistically significant differences were found between H3K27me3 expression, histopathological features of tumors, and their immunoreactivity for Sox10, claudin-1, GFAP, and Ki67. Because the classification of canine NST is not yet fully established and its correlation with the prognosis and clinical course of the disease is lacking, prospective studies with possible genetic analyses are needed to assess the true diagnostic value of H3K27me3 loss in canine NST.

• Language: English
• Keywords: dog, nerve sheath tumor, immunohistochemistry, methylation, H3K27me3, nerve sheath neoplasms
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty; MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 9 str.
• Numbering: Vol. 9, art. 921720
• PID: 20.500.12556/RUL-138597
• UDC: 636.7.09:616
• ISSN on article: 2297-1769
• DOI: 10.3389/fvets.2022.921720
• COBISS.SI-ID: 117129987
• Publication date in RUL: 02.08.2022

Record is a part of a journal

• Title: Frontiers in veterinary science
• Shortened title: Front. vet. sci.
• Publisher: Frontiers Media
• ISSN: 2297-1769
• COBISS.SI-ID: 3969402

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Projects

• Funder: Other - Other funder or multiple funders
• Funding programme: University of Pisa, Ph.D. grants

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0092
• Name: Zdravje živali, okolje in varna hrana