Antioxidant and antimicrobial biofoil based on chitosan and Japanese knotweed (Fallopia japonica, Houtt.) : rhizome bark extract

Naumoska, Katerina (author), Jug, Urška (author), Kõrge, Kristi (author), Oberlintner, Ana (author), Golob, Majda (author), Novak, Uroš (author), Vovk, Irena (author), Likozar, Blaž (author)

A 70% ethanol(aq) extract of the rhizome bark of the invasive alien plant species Japanese knotweed (JKRB) with potent (in the range of vitamin C) and stable antioxidant activity was incorporated in 1% w/v into a chitosan biofoil, which was then characterized on a lab-scale. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay confirmed the antioxidant activity of the JKRB biofoil upon contact with the food simulants A, B, C, and D1 (measured half-maximal inhibitory concentrations—IC50) and supported the Folin–Ciocalteu assay result. The migration of the antioxidant marker, (−)-epicatechin, into all food simulants (A, B, C, D1, D2, and E) was quantified using liquid chromatography hyphenated to mass spectrometry (LC-MS). Calculations showed that 1 cm2 of JKRB biofoil provided antioxidant activity to ~0.5 L of liquid food upon 1 h of contact. The JKRB biofoil demonstrated antimicrobial activity against Gram-positive bacteria. The incorporation of JKRB into the chitosan biofoil resulted in improved tensile strength from 0.75 MPa to 1.81 MPa, while elongation decreased to 28%. JKRB biofoil’s lower moisture content compared to chitosan biofoil was attributed to the formation of hydrogen bonds between chitosan biofoil and JKRB compounds, further confirmed with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The JKRB biofoil completely degraded in compost in 11 days. The future upscaled production of JKRB biofoil from biowastes for active packaging may support the fights against plastic waste, food waste, and the invasiveness of Japanese knotweed, while greatly contributing to the so-called ‘zero-waste’ strategy and the reduction in greenhouse gas emissions.

• Language: English
• Keywords: Japanese knotweed rhizome bark extract, chitosan, antioxidant biofoil, antimicrobial activity, biodegradable material, active packaging
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 22 st.
• Numbering: Vol. 11, no. 6, art. 1200
• PID: 20.500.12556/RUL-137960
• UDC: 579
• ISSN on article: 2076-3921
• DOI: 10.3390/antiox11061200
• COBISS.SI-ID: 112904195
• Publication date in RUL: 08.07.2022

Record is a part of a journal

• Title: Antioxidants
• Shortened title: Antioxidants
• Publisher: MDPI
• ISSN: 2076-3921
• COBISS.SI-ID: 522976025

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 08.07.2022

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0005
• Name: Funkcionalna živila in prehranska dopolnila

• Funder: ARRS - Slovenian Research Agency
• Project number: P2-0152
• Name: Kemijsko reakcijsko inženirstvo