Biofilm in endodontics : in vitro cultivation possibilities, sonic-, ultrasonic- and laser-assisted removal techniques and evaluation of the cleaning efficacy

Josic, Uros (author), Mazzitelli, Claudia (author), Maravic, Tatjana (author), Fidler, Aleš (author), Breschi, Lorenzo (author), Mazzoni, Annalisa (author)

Incomplete and inadequate removal of endodontic biofilm during root canal treatment often leads to the clinical failure. Over the past decade, biofilm eradication techniques, such as sonication of irrigant solutions, ultrasonic and laser devices have been investigated in laboratory settings. This review aimed to give an overview of endodontic biofilm cultivation methods described in papers which investigated sonic-, ultrasonic- and Er:Yag laser-assisted biofilm removal techniques. Furthermore, the effectiveness of these removal techniques was discussed, as well as methods used for the evaluation of the cleaning efficacy. In general, laser assisted agitation, as well as ultrasonic and sonic activation of the irrigants provide a more efficient biofilm removal compared to conventional irrigation conducted by syringe/needle. The choice of irrigant is an important factor for reducing the bacterial contamination inside the root canal, with water and saline being the least effective. Due to heterogeneity in methods among the reviewed studies, it is difficult to compare sonic-, ultrasonic- and Er:Yag laser-assisted techniques among each other and give recommendations for the most efficient method in biofilm removal. Future studies should standardize the methodology regarding biofilm cultivation and cleaning methods, root canals with complex morphology should be introduced in research, with the aim of simulating the clinical scenario more closely.

• Language: English
• Keywords: biofilm, endodontics, irrigation, sonic, ultrasonic, laser
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 19 str.
• Numbering: Vol. 14, iss. 7, art. 1334
• PID: 20.500.12556/RUL-137507
• UDC: 616.31
• ISSN on article: 2073-4360
• DOI: 10.3390/polym14071334
• COBISS.SI-ID: 103387907
• Publication date in RUL: 20.06.2022

Record is a part of a journal

• Title: Polymers
• Shortened title: Polymers
• Publisher: MDPI
• ISSN: 2073-4360
• COBISS.SI-ID: 517951257

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.04.2022

Secondary language

• Language: Slovenian
• Keywords: stomatologija, biofilm, endodontsko zdravljenje, laser, učinkovito čiščenje, čiščenje zobnih kanalov