Phi 6 bacteriophage inactivation by metal salts, metal powders, and metal surfaces

Molan, Katja (author), Rahmani, Ramin (author), Krklec, Daniel (author), Brojan, Miha (author), Stopar, David (author)

The interaction of phages with abiotic environmental surfaces is usually an understudied field of phage ecology. In this study, we investigated the virucidal potential of different metal salts, metal and ceramic powders doped with Ag and Cu ions, and newly fabricated ceramic and metal surfaces against Phi6 bacteriophage. The new materials were fabricated by spark plasma sintering (SPS) and/or selective laser melting (SLM) techniques and had different surface free energies and infiltration features. We show that inactivation of Phi6 in solutions with Ag and Cu ions can be as effective as inactivation by pH, temperature, or UV. Adding powder to Ag and Cu ion solutions decreased their virucidal effect. The newly fabricated ceramic and metal surfaces showed very good virucidal activity. In particular, 45%TiO$_2$ + 5%Ag + 45%ZrO$_2$ + 5%Cu, in addition to virus adhesion, showed virucidal and infiltration properties. The results indicate that more than 99.99% of viruses deposited on the new ceramic surface were inactivated or irreversibly attached to it.

• Language: English
• Keywords: bacteriophage (phage) Phi6, metal salts, ceramic–metal powders, metals, composite materials
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty; FS - Faculty of Mechanical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 12 str.
• Numbering: Vol. 14, iss. 2, art. 204
• PID: 20.500.12556/RUL-137070
• UDC: 578.347:615.281.8:546.3
• ISSN on article: 1999-4915
• DOI: 10.3390/v14020204
• COBISS.SI-ID: 94779907
• Publication date in RUL: 01.06.2022

Record is a part of a journal

• Title: Viruses
• Shortened title: Viruses
• Publisher: MDPI
• ISSN: 1999-4915
• COBISS.SI-ID: 517597977

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.02.2022

Secondary language

• Language: Slovenian
• Keywords: bakteriofagi, Phi 6, kovinski ioni, soli, prah, kompoziti