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Adhesion and stiffness of detached breast cancer cells in vitro : co-treatment with metformin and 2-deoxy-D-glucose induces changes related to increased metastatic potential
ID Zemljič Jokhadar, Špela (Author), ID Kokot, Gašper (Author), ID Pavlin, Mojca (Author), ID Derganc, Jure (Author)

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Abstract
Metastatic cancer cells can overcome detachment-induced cell death and can proliferate in anchorage-independent conditions. A recent study revealed that a co-treatment with two drugs that interfere with cell metabolism, metformin and 2-deoxy-D-glucose, promotes detachment of viable MDA-MB-231 breast cancer cells. In the present study, we analyzed if these detached viable MDA-MB-231 cells also exhibit other features related to cancer metastatic potential, i.e., if they are softer and more prone to adhere to epithelial cells. The cell mechanics of attached cells and floating cells were analyzed by optical tweezers and cell deformability cytometry, respectively. The adhesion was assessed on a confluent monolayer of HUVEC cells, with MDA-MB-231 cells either in static conditions or in a microfluidic flow. Additionally, to test if adhesion was affected by the state of the epithelial glycocalyx, HUVEC cells were treated with neuraminidase and tunicamycin. It was found that the treated MDA-MB-231 cells were more prone to adhere to HUVEC cells and that they were softer than the control, both in the floating state and after re-seeding to a substrate. The changes in the HUVEC glycocalyx, however, did not increase the adhesion potential of MDA-MB-231.

Language:English
Keywords:metastatic potential, adhesion to epithelial cells, optical tweezers, cell deformability cytometry
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
FE - Faculty of Electrical Engineering
Publication status:Published
Publication version:Version of Record
Year:2021
Number of pages:15 str.
Numbering:Vol. 10, iss. 9, art. 873
PID:20.500.12556/RUL-136166 This link opens in a new window
UDC:616-006
ISSN on article:2079-7737
DOI:10.3390/biology10090873 This link opens in a new window
COBISS.SI-ID:87303939 This link opens in a new window
Publication date in RUL:14.04.2022
Views:421
Downloads:86
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Record is a part of a journal

Title:Biology
Shortened title:Biology
Publisher:MDPI
ISSN:2079-7737
COBISS.SI-ID:523004441 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:04.09.2021

Secondary language

Language:Slovenian
Keywords:metastatski potencial, adhezija na epitelijske celice, optične pincete

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P1-0055
Name:Biofizika polimerov, membran, gelov, koloidov in celic

Funder:ARRS - Slovenian Research Agency
Project number:J3-6794
Name:Celična energijska presnova kot tarča za zdravljenje raka – genski in farmakološki pristop

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