Efflux pump inhibition and resistance modulation in Mycobacterium smegmatis by Peucedanum ostruthium and its coumarins

Šimunović, Katarina (author), Solnier, Julia (author), Alperth, Fabian (author), Kunert, Olaf (author), Smole Možina, Sonja (author), Bucar, Franz (author)

Antibiotic resistance is a growing problem and may become the next major global health crisis if no timely actions are taken. Mycobacterial infections are widespread and, due to antibiotic resistance, also hard to treat and a major cause of mortality. Natural compounds have the potential to increase antibiotic effectiveness due to their resistance modulatory and antimicrobial effects. In this study, Peucedanum ostruthium extracts, fractions, and isolated compounds were investigated regarding their antimicrobial and resistance-modulatory effects as well as efflux pump inhibition in Mycobacterium smegmatis. P. ostruthium extracts were found to have anti-mycobacterial potential and resistance modulating effects on ethidium bromide activity. The major antibacterial effect was attributed to ostruthin, and we found that the more lipophilic the substrate, the greater the antimicrobial effect. Imperatorin caused potent modulatory effects by interfering with the action of the major LfrA efflux pump in M. smegmatis. The plant P. ostruthuim has a complex effect on M. smegmatis, including antibacterial, efflux pump inhibition, resistance modulation, and membrane permeabilization, and its major constituents, ostruthin and imperatorin, have a distinct role in these effects. This makes P. ostruthium and its coumarins promising therapeutics to consider in the fight against drug-resistant mycobacteria.

• Language: English
• Keywords: Mycobacterium smegmatis, efflux pump inhibitor, Peucedanum ostruthium, antibacterial properties, ostruthin, imperatorin, ethidium bromide accumulation, resistance modulation
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 17 str.
• Numbering: Vol. 10, iss. 9, art. 1075
• PID: 20.500.12556/RUL-136159
• UDC: 579.24/.26:547.9:615.3
• ISSN on article: 2079-6382
• DOI: 10.3390/antibiotics10091075
• COBISS.SI-ID: 75651587
• Publication date in RUL: 14.04.2022

Record is a part of a journal

• Title: Antibiotics
• Shortened title: Antibiotics
• Publisher: MDPI
• ISSN: 2079-6382
• COBISS.SI-ID: 522975769

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 05.09.2021

Secondary language

• Language: Slovenian
• Keywords: mikobakterije, odpornost proti antibiotikom, izlivne črpalke, protimikrobne snovi