A platform approach to protein encapsulates with controllable surface chemistry

Warner, Nina (author), Osojnik Črnivec, Ilja Gasan (author), Rana, Vijay Kumar (author), Cruz, Menandro (author), Scherman, Oren A. (author)

The encapsulation of proteins into core-shell structures is a widely utilised strategy for controlling protein stability, delivery and release. Despite the recognised utility of these microstructures, however, core-shell fabrication routes are often too costly or poorly scalable to allow for industrial translation. Furthermore, many scalable routes rely upon emulsion-techniques implicating denaturing or environmentally harmful organic solvents. Herein, we investigate core-shell protein encapsulation through single-feed, aqueous spray drying: a cheap, industrially ubiquitous particle-formation technology in the absence of organic solvents. We show that an excipient’s preference for the surface of the spray dried particle is well-predicted by its hydrodynamic diameter (D$_h$) under relevant feed buffer conditions (pH and ionic strength) and that the predictive power of D$_h$ is improved when measured at the spray dryer outlet temperature compared to room temperature (R$^2$ = 0.64 vs. 0.59). Lastly, we leverage these findings to propose an adaptable design framework for fabricating core-shell protein encapsulates by single-feed aqueous spray drying.

• Language: English
• Keywords: spray dry, protein, encapsulation, formulation, excipient, core-shell, EISA
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 20 str.
• Numbering: Vol. 27, iss. 7, art. 2197
• PID: 20.500.12556/RUL-136115
• UDC: 66.047:577.112:615.014.6
• ISSN on article: 1420-3049
• DOI: 10.3390/molecules27072197
• COBISS.SI-ID: 102605059
• Publication date in RUL: 13.04.2022

Record is a part of a journal

• Title: Molecules
• Shortened title: Molecules
• Publisher: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.04.2022

Secondary language

• Language: Slovenian
• Keywords: sušenje z razprševanjem, proteini, kapsulacija, dostavni sistemi