Determination of biogenic amines in fresh fish and processed fish products using IC-MS/MS

Kočar, Drago (author), Köse, Sevim (author), Tufan, Bekir (author), Ščavničar, Andrej (author), Pompe, Matevž (author)

A new method was proposed for the determination of underivatized biogenic amines based on ion-exchange chromatography coupled with mass spectrometry detection. The method was applied to the analysis of 10 biogenic amines in fresh and processed fish products. The amines were extracted from muscle tissue with water without any additional derivative step or sample clean-up. Separation of biogenic amines was done by the IonPac (4 × 50 mm) column, applying a gradient eluent by mixing formic acid (2 mol L$^{−1}$) and Milli-Q water (formic acid concentration from 400 mM to 2 M). The results demonstrated a linear response in the range of 0.01 to 10 mg L$^{−1}$. The detection limits for the fish products ranged from 20 ng/g up to around 400 ng/g for histamine and putrescine, respectively. Spermidine and spermine showed significantly higher detection limits. This current method can be used for the determination of biogenic amines in both fresh and processed fish products for regulatory purposes and monitoring food-safety issues relating to these amines, particularly histamine. It is also a useful method for evaluation of other commercial analytical test kits and commonly used methods that are possibly affected by the food matrix due to processing or other drawbacks arising from the derivatization process.

• Language: English
• Keywords: biogenic amines, determination, fish products, histamine, ion chromatography, MS/MS
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 14 str.
• Numbering: Vol. 10, iss. 8, art. 1746
• PID: 20.500.12556/RUL-136021
• UDC: 543.544.14:543.51:664.95
• ISSN on article: 2304-8158
• DOI: 10.3390/foods10081746
• COBISS.SI-ID: 73813507
• Publication date in RUL: 07.04.2022

KOČAR, Drago, KÖSE, Sevim, TUFAN, Bekir, ŠČAVNIČAR, Andrej and POMPE, Matevž, 2021, Determination of biogenic amines in fresh fish and processed fish products using IC-MS/MS. Foods [online]. 2021. Vol. 10, no. 8,  1746. [Accessed 24 June 2025]. DOI 10.3390/foods10081746. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=136021

Record is a part of a journal

• Title: Foods
• Shortened title: Foods
• Publisher: MDPI
• ISSN: 2304-8158
• COBISS.SI-ID: 512252472

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.08.2021

Secondary language

• Language: Slovenian
• Keywords: biogeni amini, določitev, ribji izdelki, histamin, ionska kromatografija, masna spektrometrija