Effects of bioinsecticidal aegerolysin-based cytolytic complexes on non-target organisms

Panevska, Anastasija (author), Glavan, Gordana (author), Jemec Kokalj, Anita (author), Kukuljan, Veronika (author), Trobec, Tomaž (author), Žužek, Monika C. (author), Vrecl, Milka (author), Drobne, Damjana (author), Frangež, Robert (author), Sepčić, Kristina (author)

Aegerolysin proteins ostreolysin A6 (OlyA6), pleurotolysin A2 (PlyA2) and erylysin A (EryA) produced by the mushroom genus Pleurotus bind strongly to an invertebrate-specific membrane sphingolipid, and together with a protein partner pleurotolysin B (PlyB), form transmembrane pore complexes. This pore formation is the basis for the selective insecticidal activity of aegerolysin/PlyB complexes against two economically important coleopteran pests: the Colorado potato beetle and the western corn rootworm. In this study, we evaluated the toxicities of these aegerolysin/PlyB complexes using feeding tests with two ecologically important non-target arthropod species: the woodlouse and the honey bee. The mammalian toxicity of the EryA/PlyB complex was also evaluated after intravenous administration to mice. None of the aegerolysin/PlyB complexes were toxic against woodlice, but OlyA6/PlyB and PlyA2/PlyB were toxic to honeybees, with 48 h mean lethal concentrations (LC$_{50}$) of 0.22 and 0.39 mg/mL, respectively, in their food. EryA/PlyB was also tested intravenously in mice up to 3 mg/kg body mass, without showing toxicity. With no toxicity seen for EryA/PlyB for environmentally beneficial arthropods and mammals at the tested concentrations, these EryA/PlyB complexes are of particular interest for development of new bioinsecticides for control of selected coleopteran pests.

• Language: English
• Keywords: adverse effects, aegerolysins, Apis mellifera, bioinsecticides, erylysin A, MACPF protein, non-target organisms, oyster mushroom, Porcellio scaber, toxicity
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty; VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 13 str.
• Numbering: Vol. 13, iss. 7, art. 457
• PID: 20.500.12556/RUL-135736
• UDC: 577
• ISSN on article: 2072-6651
• DOI: 10.3390/toxins13070457
• COBISS.SI-ID: 68710147
• Publication date in RUL: 30.03.2022

Record is a part of a journal

• Title: Toxins
• Shortened title: Toxins
• Publisher: MDPI
• ISSN: 2072-6651
• COBISS.SI-ID: 517594649

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.07.2021

Secondary language

• Language: Slovenian
• Keywords: stranski učinki, egerolizini, Apis mellifera, bioinsekticidi, erilizin A, protein z domeno MACPF, netarčni organizmi, bukov ostrigar, Porcellio scaber, toksičnost