Your browser does not allow JavaScript!
JavaScript is necessary for the proper functioning of this website. Please enable JavaScript or use a modern browser.
Open Science Slovenia
Open Science
DiKUL
slv
|
eng
Search
Browse
New in RUL
About RUL
In numbers
Help
Sign in
Detection of antibodies against tick-borne encephalitis virus in zoo animals using non-invasive blood sampling with medicinal leeches (Hirudo medicinalis)
ID
Kvapil, Pavel
(
Author
),
ID
Kastelic, Marjan
(
Author
),
ID
Jež, Nuša
(
Author
),
ID
Sedlák, Kamil
(
Author
),
ID
Kašpárková, Nikola
(
Author
),
ID
Jelovšek, Mateja
(
Author
),
ID
Avšič-Županc, Tatjana
(
Author
),
ID
Bártová, Eva
(
Author
),
ID
Račnik, Joško
(
Author
)
PDF - Presentation file,
Download
(241,79 KB)
MD5: E8915BC9B75E4ED7766BCC471E2302AA
URL - Source URL, Visit
https://www.mdpi.com/2076-0817/10/8/952
Image galllery
Abstract
Reports on non-invasive blood sampling are limited, and there are only a few studies on using kissing bugs (Reduviidae) and medicinal leeches (Hirudo medicinalis) for hematology and biochemistry testing in various zoo animal species. The aim of this study was to evaluate the usefulness of non-invasive blood sampling with medicinal leeches for arbovirus epidemiological investigations in various animal species from one zoo collection. Medicinal leeches were manually applied on 35 animals of 11 species. Control blood samples were obtained by venipuncture of the jugular vein. Antibodies to tick-borne encephalitic virus (TBEV) were detected by using the immunoenzymatic method or an immunofluorescent assay (IFAT), depending on the animal species. One of the 35 animals (2.9%) was seropositive (Ovis aries), whereas the rest of the samples were seronegative in both methods of sampling (non-invasive by leeches vs. invasive by venipuncture). Blood sampling using medicinal leeches showed promising results. It is likely a good alternative to other more complex and invasive methods, and it can provide significant advancement in blood sampling for preventive medicine and epidemiological studies in zoo animals.
Language:
English
Keywords:
infectious disease monitoring
,
animal welfare
,
epidemiology
,
communicable diseases
,
encephalitozoonosis
,
prevention
,
control
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
MF - Faculty of Medicine
VF - Veterinary Faculty
Publication status:
Published
Publication version:
Version of Record
Year:
2021
Number of pages:
6 str.
Numbering:
Vol. 10, iss. 8, art. 952
PID:
20.500.12556/RUL-135711
UDC:
636.09:616.9
ISSN on article:
2076-0817
DOI:
10.3390/pathogens10080952
COBISS.SI-ID:
71927811
Publication date in RUL:
29.03.2022
Views:
773
Downloads:
101
Metadata:
Cite this work
Plain text
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Copy citation
Share:
Record is a part of a journal
Title:
Pathogens
Shortened title:
Pathogens
Publisher:
MDPI
ISSN:
2076-0817
COBISS.SI-ID:
523338009
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
01.08.2021
Projects
Funder:
Other - Other funder or multiple funders
Funding programme:
University of Veterinary Sciences Brno
Project number:
FVHE/Literák/ITA2020
Funder:
ARRS - Slovenian Research Agency
Project number:
P4-0092
Name:
Zdravje živali, okolje in varna hrana
Similar documents
Similar works from RUL:
Similar works from other Slovenian collections:
Back