The composition of gut microbiota varies between individuals. Recently, researchers are focusing on the studies of spore-forming bacteria from the human gut microbiota and their role in host to host transmission. In such studies, the main problem is the identification of spore-forming bacteria from the gut microbiota. Studies on the human gut microbiota are usually based on metagenic sequencing. Despite its many advantages this method also has limitations as not all microorganisms are equally well detected. In order to reduce these limitations the culturomics has been developing. The aim of our master thesis was to isolate and identify spore-forming bacteria from fecal samples and to compare them between cohabiting family members. Stool samples from four healthy individuals were used. Stool samples were treated with alcohol and inoculated on different solid media. Stool samples had also been cultivated in liquid media and then inoculated on different solid media. To identify spore-forming bacteria from human gut microbiota molecular and culture methods were used. MALDI TOF MS and 16S rRNA gene sequencing were used to identify pure cultures, and the whole bacterial community was analyzed by 16S rRNA metagenomic sequencing. We found that cultivation of faecal suspension in liquid media did not increase the number of identified bacteria from human gut microbiota. Analysis of the pure cultures showed that some bacterial species were present in all family members. Moreover, some ZOTU of the spore-forming strains were present in all family members.
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