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Bacterial cell wall material properties determine E. coli resistance to sonolysis
ID Pandur, Žiga (Author), ID Dular, Matevž (Author), ID Kostanjšek, Rok (Author), ID Stopar, David (Author)

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Abstract
The applications of bacterial sonolysis in industrial settings are plagued by the lack of the knowledge of the exact mechanism of action of sonication on bacterial cells, variable effectiveness of cavitation on bacteria, and inconsistent data of its efficiency. In this study we have systematically changed material properties of E. coli cells to probe the effect of different cell wall layers on bacterial resistance to ultrasonic irradiation (20 kHz, output power 6,73 W, horn type, 3 mm probe tip diameter, 1 ml sample volume). We have determined the rates of sonolysis decay for bacteria with compromised major capsular polymers, disrupted outer membrane, compromised peptidoglycan layer, spheroplasts, giant spheroplasts, and in bacteria with different cell physiology. The non-growing bacteria were 5-fold more resistant to sonolysis than growing bacteria. The most important bacterial cell wall structure that determined the outcome during sonication was peptidoglycan. If peptidoglycan was remodelled, weakened, or absent the cavitation was very efficient. Cells with removed peptidoglycan had sonolysis resistance equal to lipid vesicles and were extremely sensitive to sonolysis. The results suggest that bacterial physiological state as well as cell wall architecture are major determinants that influence the outcome of bacterial sonolysis.

Language:English
Keywords:viability, bacteria, Escherichia coli, sonication, cell envelope
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
FS - Faculty of Mechanical Engineering
Publication status:Published
Publication version:Version of Record
Year:2022
Number of pages:10 str.
Numbering:Vol. 83, art. 105919
PID:20.500.12556/RUL-135331 This link opens in a new window
UDC:579.23:577.352:544.57
ISSN on article:1350-4177
DOI:10.1016/j.ultsonch.2022.105919 This link opens in a new window
COBISS.SI-ID:93971971 This link opens in a new window
Publication date in RUL:08.03.2022
Views:1348
Downloads:161
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Record is a part of a journal

Title:Ultrasonics Sonochemistry
Shortened title:Ultrason. sonochem.
Publisher:Butterworth-Heinemann, Elsevier Science
ISSN:1350-4177
COBISS.SI-ID:707668 This link opens in a new window

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:18.01.2022

Secondary language

Language:Slovenian
Keywords:bakterijske celice, Escherichia coli, celična ovojnica, liza celic, ultrazvočna sonikacija, sonoliza

Projects

Funder:EC - European Commission
Funding programme:H2020
Project number:771567
Name:An investigation of the mechanisms at the interaction between cavitation bubbles and contaminants
Acronym:CABUM

Funder:ARRS - Slovenian Research Agency
Project number:P2-0401
Name:Energetsko strojništvo

Funder:ARRS - Slovenian Research Agency
Project number:P4-0116
Name:Mikrobiologija in biotehnologija živil in okolja

Funder:ARRS - Slovenian Research Agency
Project number:I0-0022
Name:Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)

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