Analysis of morphological traits, cannabinoid profiles, THCAS gene sequences, and photosynthesis in wide and narrow leaflet high-cannabidiol breeding populations of medical cannabis
ID Murovec, Jana (Author), ID Eržen, Jan Jurij (Author), ID Flajšman, Marko (Author), ID Vodnik, Dominik (Author)

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Cannabis sativa L. is one of the oldest cultivated crops, used in medicine for millennia due to therapeutic characteristics of the phytocannabinoids it contains. Its medicinal properties are highly influenced by the chemotype, that is, the ratio of the two main cannabinoids cannabidiol (CBD) and Δ-9-tetrahydrocannabinol (THC). Based on published data, the chemotype should correlate with plant morphology, genetics, and photosynthetic properties. In this work, we investigated leaf morphology, plant growth characteristics, cannabinoid profiles, THCAS gene sequences, and plant photosynthetic traits in two breeding populations of medical cannabis (MX-CBD-11 and MX-CBD-707). The populations differed significantly in morphological traits. The MX-CBD-11 plants were taller, less branched, and their leaves had narrower leaflets than the bushier, wideleaved MX-CBD-707 plants, and there were significant differences between populations in the dry biomass of different plant parts. Based on these morphological differences, MX-CBD-11 was designated as a narrow leaflet drug type or vernacular “Sativa” type, while MX-CBD-707 was classified as wide leaflet drug type or “Indica” type. Chemical characterisation revealed a discrepancy between the expected chemotypes based on plant morphology; although both populations have high CBD, within each Type II (CBD/THC intermediate) and Type III (CBD dominant) plants were detected. The THCAS gene sequence analysis clustered the plants based on their chemotypes and showed high similarity to the THCAS sequences deposited in NCBI. In silico complementary analysis, using published molecular markers for chemotype determination, showed their low discrimination power in our two populations, demonstrating the genotype dependence of the molecular markers. Basic photosynthetic traits derived from light and CO$_2$ response curves were similar in the populations. However, measurements of gas exchange under chamber conditions revealed higher stomatal conductivity and photosynthesis in MX-CBD-707 plants, which were also characterised by higher day respiration. The results of this study showed that based on visual appearance and some morphological measurements, it is not possible to determine a plant’s chemotype. Visually homogenous plants had different cannabinoid profiles and, vice versa, morphologically distinct plants contained similar CBD and THC content. The two chemotypes identified in our experimental plants therefore did not correlate with plant visual appearance, leaf morphometry, and photosynthetic properties of the populations studied. Correlation was only demonstrated with the respect to THCAS sequences, which showed great discrimination power between the chemotypes.

Keywords:Cannabis sativa L., high CBD, medical cannabis, cannabinoids, photosynthesis, respiration, THCA synthase, morphometry
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Number of pages:15 str.
Numbering:Vol. 13, art. 786161
PID:20.500.12556/RUL-135198 This link opens in a new window
ISSN on article:1664-462X
DOI:10.3389/fpls.2022.786161 This link opens in a new window
COBISS.SI-ID:99091459 This link opens in a new window
Publication date in RUL:28.02.2022
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Record is a part of a journal

Title:Frontiers in plant science
Shortened title:Front. plant sci.
Publisher:Frontiers Media
COBISS.SI-ID:3011663 This link opens in a new window


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Keywords:Cannabis sativa, medicinska konoplja, vsebnost kanabinoidov, kanabinoidi, fotosinteza, dihanje, morfometrija


Funder:Drugi - Drug financer ali več financerjev
Funding programme:MGC Pharmaceuticals Ltd.
Name:Breeding medical cannabis (Cannabis sativa L.)

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P4-0077
Name:Kmetijske rastline - genetika in sodobne tehnologije

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P4-0085

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Funding programme:Infrastructure Centre
Project number:IO-0022-0481-001
Acronym:IC RRC-AG

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