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Virome status of preclonal candidates of grapevine varieties (Vitis vinifera L.) from the Slovenian wine-growing region Primorska as determined by high-throughput sequencing
ID
Miljanić, Vanja
(
Author
),
ID
Jakše, Jernej
(
Author
),
ID
Kunej, Urban
(
Author
),
ID
Rusjan, Denis
(
Author
),
ID
Škvarč, Andreja
(
Author
),
ID
Štajner, Nataša
(
Author
)
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MD5: 29A48B0DD16D9720997525B5B095E811
URL - Source URL, Visit
https://www.frontiersin.org/articles/10.3389/fmicb.2022.830866/full
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Abstract
Diseases caused by viruses and virus-like organisms are one of the major problems in viticulture and grapevine marketing worldwide. Therefore, rapid and accurate diagnosis and identification is crucial. In this study, we used HTS of virus- and viroid-derived small RNAs to determine the virome status of Slovenian preclonal candidates of autochthonous and local grapevine varieties (Vitis vinifera L.). The method applied to the studied vines revealed the presence of nine viruses and two viroids. All viral entities were validated and more than 160 Sanger sequences were generated and deposited in NCBI. In addition, a complete description into the co-infections in each plant studied was obtained. No vine was found to be virus- and viroid-free, and no vine was found to be infected with only one virus or viroid, while the highest number of viral entities in a plant was eight.
Language:
English
Keywords:
Vitis vinifera L.
,
preclonal candidates
,
HTS
,
viruses
,
viroids
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
BF - Biotechnical Faculty
Publication status:
Published
Publication version:
Version of Record
Year:
2022
Number of pages:
11 str.
Numbering:
Vol. 13, art. 830866
PID:
20.500.12556/RUL-135073
UDC:
634.8
ISSN on article:
1664-302X
DOI:
10.3389/fmicb.2022.830866
COBISS.SI-ID:
98211843
Publication date in RUL:
21.02.2022
Views:
824
Downloads:
150
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Record is a part of a journal
Title:
Frontiers in microbiology
Shortened title:
Front. microbiol.
Publisher:
Frontiers Research Foundation
ISSN:
1664-302X
COBISS.SI-ID:
4146296
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
vinska trta
,
sorte
,
klonska selekcija
,
predklonski kandidati
,
sekvenciranje
,
virusi
,
viroidi
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P4-0077
Name:
Kmetijske rastline - genetika in sodobne tehnologije
Funder:
Other - Other funder or multiple funders
Funding programme:
Public Scholarship, Development, Disability and Maintenance Fund of the Republic of Slovenia, Scholarship for nationals of Western Balkans to postgraduate study in Slovenia
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