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Pomen vodnega režima in vsebnosti silicija na razgradnjo in sestavo mikrobnih združb opada navadnega trsta : magistrsko delo
ID Samardžić, Tijana (Author), ID Likar, Matevž (Mentor) More about this mentor... This link opens in a new window, ID Vogel-Mikuš, Katarina (Co-mentor), ID Regvar, Marjana (Reviewer), ID Germ, Mateja (Thesis defence commission member)

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Abstract
Ker imajo glive in bakterije ključno vlogo med procesom razgradnje, smo preučevali, kako vsebnost silicija (Si) in sprememba vodnega režima vplivata na strukturo in pestrost mikrobnih združb. Za določanje vpliva vodnega režima na potek razgradnje, smo liste navadnega trsta različne starosti in iz različnih geografskih lokacij izpostavili razgradnji na suhi in mokri mikrolokaciji. Nato smo z uporabo visokozmogljivega sekvenciranja analizirali strukturo in diverziteto mikrobov ter ugotovili, da glivne operacijske taksonomske enote (ang. operational taxonomic unit–OTU) večinoma sodijo v deblo Ascomycota (95,7 %) in da so med njimi najbolj številni OTU s saprotrofskim načinom prehranjevanja (48,6%). Pokazali smo, da na pestrost mikrobnih združb najbolj vpliva vodni režim. Pestrost bakterijskih združb se z razgradnjo poveča. Ugotovili smo pozitivno korelacijo (R=0,75; p<0,001) med vsebnostjo Si in pestrostjo združb gliv v vzorcih, ne pa tudi združb bakterij. S rezultati, pridobljeni z uporabo metode XRF smo pokazali, da na vsebnost Si v vzorcih vplivajo: starost listov, geografska lokacija in vodni režim. Pokazali smo tudi težnjo upada vsebnosti makronutrientov po razgradnji. Nasprotno se je vsebnost Fe zvišala pri vzorcih, izpostavljenih razgradnji na mokri mikrolokaciji. Vsebnost Zn in Mn se je razlikovala med vzorci iz različnih geografskih lokacij, izpostavljenih razgradnji na suhi mikrolokaciji. S spektroskopsko metodo FTIR smo ugotovili značilen upad celuloznih komponent pri vzorcih, izpostavljenih razgradnji na mokri mikrolokaciji. Z vidika biokemijske sestave opada po razgradnji je razvidno, da je bila razgradnja pospešena na mokri mikrolokaciji, ki je sovpadla z večjo diverziteto bakterijskih združb.

Language:Slovenian
Keywords:silicij, vodni režim, razgradnja, mikrobne združbe, navadni trst
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Place of publishing:Ljubljana
Publisher:[T. Samardžić]
Year:2022
Number of pages:XI, 68 f.
PID:20.500.12556/RUL-134909 This link opens in a new window
UDC:579:582.542(043.2)
COBISS.SI-ID:103834371 This link opens in a new window
Publication date in RUL:11.02.2022
Views:577
Downloads:61
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Secondary language

Language:English
Title:The effect of silicon content and water regime on degradation and microbial communities on common reeds' litter : magistrski študij - 2. stopnja
Abstract:
Since fungi and bacteria play major role during the decomposition process, we analyzed silicon (Si) content and water regime as factors affecting microbial structure and diversity. To assess how water regime affects decomposition, we exposed common reed's leaf samples varying in age and originating from two different geographical locations to decomposition process in wet and dry microlocations. Using high throughput sequencing we analyzed microbial structure and diversity and showed that most of the fungal operational taxonomic units (OTU) are saprotrophs (48,6%) belonging to the phylum Ascomycota (95,7%). We showed that the water regime significantly affects microbial diversity. Bacterial diversity increases during the decomposition. Also we displayed positive correlation (R=0,75; p<0,001) between Si content and fungal diversity, which was not the case for bacterial diversity. Results obtained using XRF method showed significant effect of leaf age, geographical location and water regime on Si content. Additionally we showed macronutrient content decrease during the decomposition. On the other side, Fe content increased in samples exposed in wet microlocation while we displayed distinctive pattern of Zn and Mn content between samples from different geographical locations exposed to decomposition in dry microlocation. Using FTIR method we measured sample organic compound content and showed cellulose components decrease in samples exposed to decomposition in wet microlocation. From the aspect of biochemical properties, decomposition was promoted in wet microlocation, which is consistent with higher bacterial diversity.

Keywords:silicon, water regime, decomposition, microbial communities, common reed

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