Genomic characterization of the Istrian shorthaired hound

Djurkin Kušec, Ivona (author), Bošković, Ivica (author), Zorc, Minja (author), Gvozdanović, Kristina (author), Škorput, Dubravko (author), Dovč, Peter (author), Kušec, Goran (author)

Istrian shorthaired hound is an old indigenous Croatian dog breed with historical traces of its origin, which date back to the 14th century. Due to its intelligence and great hunting abilities, it is considered an excellent hunting dog. Despite its ancient origin, there is no data on genetic diversity, population structure, and degree of inbreeding that could be used for advanced management and conservation of this breed. Our study aimed to provide a high-resolution population structure of the Istrian shorthaired hound using a 220K HD SNP array, to compare the obtained data with the genealogical records and to place the breed in a broader context of world dog populations. Relatively high population size and low inbreeding coefficient estimated from genealogical data indicate a preserved genetic diversity in this breed. The principle component analysis, the NeighborNet network, and TreeMix were used to determine the genetic relationship between the Istrian shorthaired hound and other breeds. The Istrian shorthaired hound was found to be genetically related to Italian hunting dogs sharing the same branch with the Segugio Italiano a Pelo Raso and Segugio Italiano a Pelo Forte. The ADMIXTURE analysis indicated that the Istrian shorthaired hound could be involved in the development of some other hunting dog breeds. The estimated effective population size (Ne) based on SNP data was similar to Ne calculated from genealogical data indicating the absence of bottlenecks and well-balanced use of breeding animals. The low genomic inbreeding coefficient, together with the higher number of short runs of homozygosity, observed in the Istrian shorthaired hound, confirms the ancient origin of the breed based on historical documents. The analysis of selective sweeps identified genomic regions with the strongest selection signals in the vicinity of the genes associated with cognitive performance and behavior. Genome analysis proved to be a useful tool for estimating population parameters and can be implemented in the conservation plan for this indigenous breed.

• Language: English
• Keywords: Istrian shorthaired hound, genetic diversity, population structure, genealogical data, SNP array
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 20 str.
• Numbering: Vol. 10, iss. 11, art. 2013
• PID: 20.500.12556/RUL-134705
• UDC: 636.7:575
• ISSN on article: 2076-2615
• DOI: 10.3390/ani10112013
• COBISS.SI-ID: 35865347
• Publication date in RUL: 27.01.2022

Record is a part of a journal

• Title: Animals
• Shortened title: Animals
• Publisher: MDPI
• ISSN: 2076-2615
• COBISS.SI-ID: 519120409

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.11.2020

Secondary language

• Language: Slovenian
• Keywords: psi, pasme, istrski kratkodlaki gonič, genetika, genomska karakterizacija

Projects

• Funder: Other - Other funder or multiple funders
• Funding programme: Josip Juraj Strossmayer University of Osijek
• Project number: UNIOS-ZUP 2018-92

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0220
• Name: Primerjalna genomika in genomska biodiverziteta