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Analysis of the direct and indirect effects of nanoparticle exposure on microglial and neuronal cells in vitro
ID Lojk, Jasna (Author), ID Babič, Lea (Author), ID Sušjan-Leite, Petra (Author), ID Bregar, Vladimir Boštjan (Author), ID Pavlin, Mojca (Author), ID Hafner Bratkovič, Iva (Author), ID Veranič, Peter (Author)

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Abstract
Environmental or biomedical exposure to nanoparticles (NPs) can results in translocation and accumulation of NPs in the brain, which can lead to health-related problems. NPs have been shown to induce toxicity to neuronal cells through several direct mechanisms, but only a few studies have also explored the indirect effects of NPs, through consequences due to the exposure of neighboring cells to NPs. In this study, we analysed possible direct and indirect effects of NPs (polyacrylic acid (PAA) coated cobalt ferrite NP, TiO$_2$ P25 and maghemite NPs) on immortalized mouse microglial cells and differentiated CAD mouse neuronal cells in monoculture (direct toxicity) or in transwell co-culture system (indirect toxicity). We showed that although the low NP concentrations (2–25 µg/mL) did not induce changes in cell viability, cytokine secretion or NF-κB activation of microglial cells, even low NP concentrations of 10 µg/mL can affect the cells and change their secretion of protein stress mediators. These can in turn influence neuronal cells in indirect exposure model. Indirect toxicity of NPs is an important and not adequately assessed mechanism of NP toxicity, since it not only affects cells on the exposure sites, but through secretion of signaling mediators, can also affect cells that do not come in direct contact with NPs.

Language:English
Keywords:nanoparticle, neurotoxicity, cytokine secretion, ROS, microglia, co-culture
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:FE - Faculty of Electrical Engineering
MF - Faculty of Medicine
Publication status:Published
Publication version:Version of Record
Year:2020
Number of pages:15 str.
Numbering:Vol. 21, iss. 19, art. 7030
PID:20.500.12556/RUL-134357 This link opens in a new window
UDC:61
ISSN on article:1422-0067
DOI:10.3390/ijms21197030 This link opens in a new window
COBISS.SI-ID:30614531 This link opens in a new window
Publication date in RUL:10.01.2022
Views:714
Downloads:121
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Record is a part of a journal

Title:International journal of molecular sciences
Shortened title:Int. j. mol. sci.
Publisher:MDPI
ISSN:1422-0067
COBISS.SI-ID:2779162 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:01.10.2020

Secondary language

Language:Slovenian
Keywords:nanodelci, nevrotoksičnost, izločanje citokinov

Projects

Funder:ARRS - Slovenian Research Agency
Project number:Z4-8229
Name:Vpliv različnih tipov nanodelcev na živčne celice in makrofage – analiza kratkoročnih in dolgoročnih posledic na celični stres, diferenciacijo in celično signaliziranje

Funder:ARRS - Slovenian Research Agency
Project number:J7-7424
Name:Analiza možnih škodljivih učinkov nanodelcev in spremljajočih mehanizmov – od fizikalno-kemijske ter in vitro karakterizacije do aktivacije prirojenega imunskega sistema

Funder:ARRS - Slovenian Research Agency
Project number:P1-0055
Name:Biofizika polimerov, membran, gelov, koloidov in celic

Funder:ARRS - Slovenian Research Agency
Funding programme:Young Researcher

Funder:ARRS - Slovenian Research Agency
Project number:I0-0022
Name:Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)

Funder:Other - Other funder or multiple funders
Funding programme:COST
Project number:CA16122
Acronym:BIONECA

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