A comprehensive study of cutaneous fibropapillomatosis in free-ranging roe deer (Capreolus capreolus) and red deer (Cervus elaphus) : from clinical manifestations to whole-genome sequencing of papillomaviruses

Kmetec, Jernej (author), Kuhar, Urška (author), Grilc Fajfar, Aleksandra (author), Žele-Vengušt, Diana (author), Vengušt, Gorazd (author)

Papillomaviruses (PVs) are an extremely large group of viruses that cause skin and mucosa infections in humans and various animals. In roe deer and red deer, most PVs belong to the Deltapapillomavirus genus and cause neoplastic changes that are generally described as fibropapillomas. Despite the wide distribution of roe and red deer throughout Europe and beyond, the data in the scientific literature regarding the widespread distribution of PVs and the genetic variability of PV genomes in these species are rather scarce. This study describes cutaneous fibropapillomatosis cases in roe and red deer with clinical manifestations that are typical of infections with PVs. In all cases, the presence of PV DNA was confirmed using PCR, followed by Sanger sequencing of the partial L1 gene. The complete PV genomes were determined in all the investigated samples using next-generation sequencing technology, revealing infections of roe deer with the CcaPV1-type and red deer with the CePV1v-type variant. A comparison of the complete CcaPV1-type and CePV1v-type variant genome sequences reported here with already available complete genome sequences in GenBank revealed their great genetic stability across time and space.

• Language: English
• Keywords: papillomavirus, fibropapillomatosis, red deer, roe deer, genome, NGS, Papillomaviridae, deer
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 14 str.
• Numbering: Vol. 12, iss. 9, art. 1001
• PID: 20.500.12556/RUL-134269
• UDC: 578
• ISSN on article: 1999-4915
• DOI: 10.3390/v12091001
• COBISS.SI-ID: 27616515
• Publication date in RUL: 04.01.2022

Record is a part of a journal

• Title: Viruses
• Shortened title: Viruses
• Publisher: MDPI
• ISSN: 1999-4915
• COBISS.SI-ID: 517597977

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 08.09.2020