Molecular study of selected taxonomically critical taxa of the genus Iris L. from the broader Alpine-Dinaric area

Weber, Tim (author), Jakše, Jernej (author), Sladonja, Barbara (author), Hruševar, Dario (author), Landeka, Nediljko (author), Brana, Slavko (author), Bohanec, Borut (author), Milović, Milenko (author), Vladović, Dalibor (author), Mitić, Božena (author), Poljuha, Danijela (author)

Some wild, morphologically diverse taxa of the genus Iris in the broad Alpine-Dinaric area have never been explored molecularly, and/or have ambiguous systematic status. The main aims of our research were to perform a molecular study of critical Iris taxa from that area (especially a narrow endemic accepted species I. adriatica, for which we also analysed genome size) and to explore the contribution of eight microsatellites and highly variable chloroplast DNA (ndhJ, rpoC1) markers to the understanding of the Iris taxa taxonomy and phylogeny. Both the microsatellite-based UPGMA and plastid markers-based maximum likelihood analysis discriminated three main clusters in the set of 32 analysed samples, which correspond well to the lower taxonomic categories of the genus, and support separate status of ambiguous regional taxa (e.g., I. sibirica subsp. erirrhiza, I. x croatica and I. x rotschildii). The first molecular data on I. adriatica revealed its genome size (2C = 12.639 ± 0.202 pg) and indicated the existence of ecotypes. For future molecular characterisation of the genus we recommend the utilisation of microsatellite markers supplemented with a combination of plastid markers.

• Language: English
• Keywords: Iridaceae, Europe, chloroplast DNA, microsatellites, phylogeny, taxonomy
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 16 str.
• Numbering: Vol. 9, iss. 9, art. 1229
• PID: 20.500.12556/RUL-134235
• UDC: 577.2
• ISSN on article: 2223-7747
• DOI: 10.3390/plants9091229
• COBISS.SI-ID: 29832451
• Publication date in RUL: 03.01.2022

Record is a part of a journal

• Title: Plants
• Shortened title: Plants
• Publisher: MDPI
• ISSN: 2223-7747
• COBISS.SI-ID: 523345433

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 18.09.2020

Secondary language

• Language: Slovenian
• Keywords: Iridaceae, mikrosatelitski markerji, filogenija, molekularna biologija

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0077
• Name: Kmetijske rastline - genetika in sodobne tehnologije

• Funder: Other - Other funder or multiple funders
• Funding programme: University of Zagreb
• Project number: 106-F20-00025